In vitro exposure to highly cytopathic HIV-1 X4 strains increases expression of mucosa-associated integrins on CD4(+) T cells.

Abstract

To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(IIIB) strain or with X4/SI or CCR5-tropic (R5)/non-SI (NSI) primary human isolates. Flow cytometric analyses of CD4(+) T cells from cultures infected with HIV-1(IIIB) and one X4/SI primary HIV-1 isolate revealed a significant increase in surface expression of alpha 4 beta 7 and alpha E beta 7 12 days after infection. L-selectin expression was not significantly affected on CD4(+) T cells. However, infection with another X4/SI and two R5/NSI primary HIV-1 isolates did not significantly alter homing receptor expression on CD4(+) T cells. Since a higher degree of CD4 cytopathicity occurred in those cultures having increased integrin expression, these data suggest that significantly altered mucosal homing receptor expression on CD4(+) T cells may result as a "bystander" effect after infection with some cytopathic isolates of HIV-1.

• Authors: J E Cummins Jr, W J Bunn, S D Hall, H H Donze, J Mestecky, S Jackson
• Journal: Virology (Virology) Vol. 280 Issue 2 Pg. 262-72 (Feb 15 2001) ISSN: 0042-6822 [Print] United States
• PMID: 11162840 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2001 Academic Press.

Chemical References

• Integrins
• integrin alpha4beta7
• integrin alphaEbeta7
• L-Selectin

Topics

• CD4-Positive T-Lymphocytes (metabolism, virology)
• Cells, Cultured
• Cytopathogenic Effect, Viral
• HIV Infections (immunology)
• HIV-1 (immunology, pathogenicity, physiology)
• Humans
• Integrins (biosynthesis)
• L-Selectin (biosynthesis)
• Mucous Membrane (metabolism)