Abstract | BACKGROUND: AIM: to assess the effect of glutamine pre-treatment in vivo and in vitro on cytokine production by intestinal mucosa. METHODS: nine fasted volunteers received either enteral glutamine or saline over 6 h in a cross-over design. Duodenal biopsies were cultured for 24 h with or without glutamine. Cytokine content of culture media was analysed by ELISA, and the expression of cytokine mRNA in biopsies was assessed by semi-quantitative RT-PCR. RESULTS: CONCLUSIONS:
glutamine reduces pro-inflammatory cytokine production by human intestinal mucosa, probably by a post-transcriptional pathway. Glutamine could be useful to modulate inflammatory conditions with imbalanced cytokine production.
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Authors | M Coëffier, O Miralles-Barrachina, F Le Pessot, O Lalaude, M Daveau, A Lavoinne, E Lerebours, P Déchelotte |
Journal | Cytokine
(Cytokine)
Vol. 13
Issue 3
Pg. 148-54
(Feb 07 2001)
ISSN: 1043-4666 [Print] England |
PMID | 11161457
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Cytokines
- Interleukin-6
- Interleukin-8
- RNA, Messenger
- Glutamine
- Interleukin-4
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Topics |
- Adolescent
- Adult
- Cytokines
(biosynthesis, genetics, metabolism)
- Duodenum
(drug effects, metabolism)
- Glutamine
(administration & dosage, pharmacology)
- Humans
- Infusions, Parenteral
- Interleukin-4
(biosynthesis, genetics, metabolism)
- Interleukin-6
(biosynthesis, genetics, metabolism)
- Interleukin-8
(biosynthesis, genetics, metabolism)
- Intestinal Mucosa
(drug effects, metabolism)
- Organ Culture Techniques
- RNA, Messenger
(biosynthesis, genetics)
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