Expression of Mig (monokine induced by interferon-gamma) is important in T lymphocyte recruitment and host defense following viral infection of the central nervous system.

Abstract

Induction of a Th1 immune response against viral infection of the CNS is important in contributing to viral clearance. The present studies demonstrate a role for the T cell chemoattractant chemokine Mig (monokine induced by IFN-gamma) in contributing to a Th1 response against mouse hepatitis virus infection of the CNS. Analysis of the kinetics of Mig expression revealed mRNA transcripts present at days 7 and 12 postinfection (p.i.) but not early (day 2) or late (day 35) in the infection. To determine functional significance, mouse hepatitis virus-infected mice were treated with anti-Mig antisera, and the severity of disease was evaluated. Such treatment resulted in a marked increase in mortality that correlated with a >3 log increase in viral burden within the brains as compared with control mice treated with normal rabbit serum. Anti-Mig-treated mice displayed a significant decrease (p < 0.005) in CD4(+) and CD8(+) T cell recruitment into the CNS as compared with normal rabbit serum-treated mice. In addition, anti-Mig treatment resulted in a significant decrease (p < 0.05) in levels of IFN-gamma and IFN-beta that coincided with increased (p < 0.02) expression of the anti-inflammatory Th2 cytokine IL-10 within the CNS. Collectively, these data indicate that Mig is important in contributing to host defense by promoting a protective Th1 response against viral infection of the CNS.

• Authors: M T Liu, D Armstrong, T A Hamilton, T E Lane
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 166 Issue 3 Pg. 1790-5 (Feb 01 2001) ISSN: 0022-1767 [Print] United States
• PMID: 11160225 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• CXCL9 protein, human
• Chemokine CXCL9
• Chemokines, CXC
• Cytokines
• Immune Sera
• Intercellular Signaling Peptides and Proteins
• Interferon-gamma

Topics

• Amino Acid Sequence
• Animals
• Cell Movement (immunology)
• Chemokine CXCL9
• Chemokines, CXC (biosynthesis, immunology)
• Coronavirus Infections (immunology, mortality, pathology, therapy)
• Cytokines (metabolism)
• Encephalitis, Viral (immunology, mortality, pathology)
• Immune Sera (administration & dosage)
• Immunity, Innate
• Injections, Intraperitoneal
• Intercellular Signaling Peptides and Proteins
• Interferon-gamma (physiology)
• Male
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data
• Murine hepatitis virus (immunology)
• T-Lymphocytes (immunology, metabolism, pathology)
• Tumor Cells, Cultured