Hemorrhage and
thrombosis are associated with major vascular and trauma surgery. Release of
heparinoids and thrombotic mediators may contribute to these complications and have been described in rabbits after aortic occlusion-reperfusion. We hypothesized that the resuscitative fluid used could reduce
heparinoid and thrombotic mediator release after aortic occlusion-reperfusion in rabbits as assessed by thromboelastographic variables (R, reaction time; alpha, angle; and G, a measure of clot strength). Anesthetized rabbits were administered
lactated Ringer's solution (n = 8) or
PentaLyte (n = 8) at reperfusion after 30 min of
ischemia. Blood was obtained before
ischemia and after 30 min of reperfusion for thromboelastography under four conditions: 1) unmodified sample, 2) platelet inhibition, 3)
heparinase, and 4) platelet inhibition and
heparinase. During reperfusion, unmodified samples demonstrated a significant increase in R and decrease in alpha and G that was not affected by
PentaLyte. In the presence of
heparinase, no significant fluid-specific thromboelastographic differences were noted. However, thrombotic mediator release (discerned by a decrease in R and an increase in alpha) during reperfusion in samples with platelet inhibition and
heparinase was significantly attenuated by
PentaLyte.
PentaLyte administration does not decrease
heparinoid release but does decrease thrombotic mediator release after aortic occlusion-reperfusion.