Rapid isolation of tissue-specific genes from rat kidney.

Abstract

A systematic effort to isolate kidney-specific genes was performed using recently described PCR-select methodology. Using this technique, a kidney-specific mini-gene library was generated and a number of kidney-specific genes that share significant homology to previously characterized kidney genes from rats and other species were isolated. These included three renal-specific transporters (an ADH water channel, the anion transporters RST and ROAT1), a cell adhesion molecule (K-cadherin) and a kidney-specific protein upregulated in renal carcinoma (DD96). In addition, we isolated two novel genes from a rat kidney. One of the genes shares limited homology to rat profilin-1 while the other did not share any similarity to genes in the Genbank. Northern blot analysis revealed that the mRNA for each of these genes is expressed in a highly kidney-restricted fashion. Our results suggested that tissue-specific genes can be rapidly isolated and characterized using PCR-select techniques and this methodology may be generally applicable to isolate specific genes from a variety of tissues.

• Authors: E Hu, Z Chen, T A Fredrickson, N Spurr, S Gentle, M Sims, Y Zhu, W Halsey, Van Horn S, J Mao, G M Sathe, D P Brooks
• Journal: Experimental nephrology (Exp Nephrol) 2001 Mar-Apr Vol. 9 Issue 2 Pg. 156-64 ISSN: 1018-7782 [Print] Switzerland
• PMID: 11150865 (Publication Type: Journal Article)
• Copyright: Copyright 2001 S. Karger AG, Basel

Chemical References

• DNA, Complementary

Topics

• Animals
• Base Sequence (genetics)
• Cloning, Molecular
• DNA, Complementary (genetics)
• Gene Expression
• Kidney (physiology)
• Molecular Sequence Data
• Polymerase Chain Reaction (methods)
• Rats
• Rats, Sprague-Dawley
• Time Factors