Abstract | PURPOSE: METHODS: ABCV (12 mg/kg wt) and AmB(DOC) (2 mg/kg wt) were intravenously administered to normal and A. fumigatus infected mice. The concentration of amphotericin B in plasma and other organs was determined at different time points. RESULTS: It was observed that ABCV had a significantly different pharmacokinetic profile compared to conventional amphotericin B. In comparison to AmB(DOC) significantly lower levels of amphotericin B were observed in kidneys and plasma, the major target organs of toxicity. Animals receiving ABCV demonstrated high levels of amphotericin B in liver (38% retention till 48 h) and spleen (2.6% retention till 48 h) in comparison to AmB(DOC) (7.3% and 0.21% retention in liver and spleen respectively till 48 h). Biodistribution studies of ABCV in infected mice demonstrated that there was a moderate enhancement in levels of amphotericin B in liver, spleen, lungs and kidneys as compared to normal mice and the plasma levels were reduced. However, such observations were not made after AmB(DOC) administration to infected mice except for kidneys in which there was a marked increase in uptake as compared to normal mice. CONCLUSIONS: Our results suggest that prolonged retention of high concentrations of ABCV in reticuloendothelial system organs is the reason for its reduced toxicity. Enhanced localization of the drug at the infected site may lead to improvement in therapeutic efficacy.
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Authors | S Saxena, P C Ghosh |
Journal | Pharmaceutical research
(Pharm Res)
Vol. 17
Issue 10
Pg. 1236-42
(Oct 2000)
ISSN: 0724-8741 [Print] United States |
PMID | 11145229
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antifungal Agents
- Buffers
- Cholesterol Esters
- Detergents
- Drug Carriers
- Suspensions
- Deoxycholic Acid
- Amphotericin B
- cholesteryl succinate
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Topics |
- Amphotericin B
(administration & dosage, blood, pharmacokinetics)
- Animals
- Antifungal Agents
(administration & dosage, blood, pharmacokinetics)
- Aspergillosis
(blood, drug therapy, metabolism)
- Aspergillus fumigatus
- Buffers
- Cholesterol Esters
(administration & dosage, blood, pharmacokinetics)
- Deoxycholic Acid
(administration & dosage)
- Detergents
(administration & dosage)
- Drug Carriers
- Drug Stability
- Male
- Mice
- Mice, Inbred BALB C
- Suspensions
- Tissue Distribution
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