Inhibitory effects of antisense oligonucleotides on the expression of procollagen type III gene in mouse hepatic stellate cells transformed by simian virus 40.

Abstract	The effects of phosphorothioate antisense oligonucleotides (ASO), complementary to the AUG start region, the junctional region of the intron and exon, and to exon of the procollagen type III gene, were investigated in a mouse hepatic stellate cell (HSC) line transformed by the simian virus 40 gene, SV68c-IS cells. ASO were transfected by lipofection. Immunohistochemistry, western and northern blotting showed inhibitory effects on procollagen type III gene expression by ASO that were complementary to the AUG start region and the junctional region of the intron and exon 2. However, ASO complementary to the exon 2 and 3, junctional region of the intron and exon 3, and sense oligonucleotides complementary to each ASO did not show any inhibitory effects. The effects of ASO complementary to the AUG start region were greater than those of ASO complementary to the junctional region. The effects of ASO were transient and a large amount of ASO was required to induce inhibitory effects without lipofection. ASO were effective in inhibiting the expression of the procollagen type III gene in the HSC which is well known to play a critical role in liver fibrosis.
Authors	S Horie, Y Kitamura, H Kawasaki, T Terada
Journal	Pathology international (Pathol Int) Vol. 50 Issue 12 Pg. 937-44 (Dec 2000) ISSN: 1320-5463 [Print] Australia
PMID	11123759 (Publication Type: Journal Article)
Chemical References	Actins Antigens, Viral, Tumor DNA, Antisense Desmin Glial Fibrillary Acidic Protein Procollagen RNA, Messenger
Topics	Actins (analysis) Animals Antigens, Viral, Tumor (analysis) Blotting, Northern Blotting, Western Cell Division (drug effects) Cell Line, Transformed Cell Transformation, Viral DNA, Antisense (genetics, pharmacology) Desmin (analysis) Dose-Response Relationship, Drug Gene Expression Regulation (drug effects) Glial Fibrillary Acidic Protein (analysis) Immunohistochemistry Liver (chemistry, cytology, virology) Mice Muscle, Smooth (chemistry) Procollagen (genetics, metabolism) RNA, Messenger (drug effects, genetics, metabolism) Simian virus 40 (growth & development) Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!