NMDA receptor mediated changes in IGF-II gene expression in the rat brain after injury and the possible role of nitric oxide.

Abstract

This study was undertaken in order to investigate the role of insulin-like growth factor (IGF)-II, c-fos, N-methyl-D-aspartate (NMDA) receptors, and nNOS in the cellular processes following a penetrating brain injury. IGF-II mRNA levels, as determined by Northern analysis, were decreased at 4, 8, and 24 h after brain injury, in the lesioned, compared to the contralateral intact hemisphere. Forty-eight and 72 h after the injury, there was no difference between the lesioned and the contralateral intact hemisphere in IGF-II mRNA levels. c-fos mRNA levels followed a parallel, but opposite course: They were increased at 4, 8 and 24 h after the injury, while at 48 and 72 h c-fos mRNA levels in the lesioned hemisphere did not differ from those in the intact. Administration of MK-801 reversed the injury-induced decrease in IGF-II mRNA levels. Administration of MK-801 resulted in an increase in IGF-II mRNA in both the intact and the lesioned hemispheres. Brain injury resulted in an increase in nNOS immunopositive cells in the hippocampal formation, which was detectable at 4 and 12, but not 48 h after the injury. These results suggest that IGF-II, c-fos, NMDA receptors and nNOS are involved in the cellular responses to brain injury.

• Authors: M Giannakopoulou, M Mansour, E Kazanis, E Bozas, H Philpipidis, F Stylianopoulou
• Journal: Neuropathology and applied neurobiology (Neuropathol Appl Neurobiol) Vol. 26 Issue 6 Pg. 513-21 (Dec 2000) ISSN: 0305-1846 [Print] England
• PMID: 11123717 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Excitatory Amino Acid Antagonists
• RNA, Messenger
• Receptors, N-Methyl-D-Aspartate
• Nitric Oxide
• Insulin-Like Growth Factor II
• Dizocilpine Maleate
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type I
• Nos1 protein, rat

Topics

• Animals
• Brain Injuries (enzymology, genetics, pathology)
• Dizocilpine Maleate (pharmacology)
• Excitatory Amino Acid Antagonists (pharmacology)
• Gene Expression (physiology)
• Hippocampus (enzymology, pathology)
• Immunohistochemistry
• Insulin-Like Growth Factor II (genetics)
• Male
• Neurons (enzymology)
• Nitric Oxide (physiology)
• Nitric Oxide Synthase (metabolism)
• Nitric Oxide Synthase Type I
• RNA, Messenger (metabolism)
• Rats
• Rats, Wistar
• Receptors, N-Methyl-D-Aspartate (physiology)
• Time Factors