A moderate and unspecific release of cysteinyl leukotrienes by aspirin from peripheral blood leucocytes precludes its value for aspirin sensitivity testing in asthma.

Aspirin-induced asthma (AIA) is a clinical syndrome related to cysteinyl leukotriene overproduction in airways. The confirmation of the diagnosis requires inconvenient provocation tests with acetyl salicylic acid (ASA). A study was performed to evaluate whether measurement in vitro of cysteinyl leukotrienes (cys-LTs) release by isolated peripheral blood leucocytes, stimulated with ASA, can be of use for diagnosis of AIA. A cellular allergen stimulation test, CAST, was adapted to measure leukotriene release from leucocytes of 32 aspirin-tolerant and 26 aspirin-intolerant asthmatics. The cells were stimulated with Lys-ASA, N-formyl-methionyl-leucyl-phenylalanine (fMLP), or both fMLP and Lys-ASA, in a buffer containing IL-3, and results compared with human leukaemia cell line (Hl-60) response to Lys-ASA. Cys-LTs were measured in cell supernatant fluids by ELISA. ASA had a rather week stimulatory effect on cys-LTs release in both groups of patients. Contrary to some previous studies, no significant differences were found between cys-LTs release by leucocytes from AIA and ATA, or by differentiated Hl-60 cells. Measurement of cysteinyl-leukotriene release by peripheral blood leucocytes pre-treated with aspirin has no value for diagnosis of AIA.
AuthorsM Pierzchalska, L Mastalerz, M Sanak, M Zazula, A Szczeklik
JournalClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 30 Issue 12 Pg. 1785-91 (Dec 2000) ISSN: 0954-7894 [Print] ENGLAND
PMID11122218 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Leukotrienes
  • N-Formylmethionine Leucyl-Phenylalanine
  • Aspirin
  • Adult
  • Aspirin (adverse effects, pharmacology)
  • Asthma (blood, chemically induced)
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • HL-60 Cells (drug effects, metabolism)
  • Humans
  • Leukocytes (drug effects, metabolism)
  • Leukotrienes (analysis)
  • Male
  • N-Formylmethionine Leucyl-Phenylalanine (pharmacology)

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