Abstract |
Aspirin-induced asthma (AIA) is a clinical syndrome related to cysteinyl leukotriene overproduction in airways. The confirmation of the diagnosis requires inconvenient provocation tests with acetyl salicylic acid (ASA). A study was performed to evaluate whether measurement in vitro of cysteinyl leukotrienes (cys-LTs) release by isolated peripheral blood leucocytes, stimulated with ASA, can be of use for diagnosis of AIA. A cellular allergen stimulation test, CAST, was adapted to measure leukotriene release from leucocytes of 32 aspirin-tolerant and 26 aspirin-intolerant asthmatics. The cells were stimulated with Lys-ASA, N-formyl-methionyl-leucyl-phenylalanine (fMLP), or both fMLP and Lys-ASA, in a buffer containing IL-3, and results compared with human leukaemia cell line (Hl-60) response to Lys-ASA. Cys-LTs were measured in cell supernatant fluids by ELISA. ASA had a rather week stimulatory effect on cys-LTs release in both groups of patients. Contrary to some previous studies, no significant differences were found between cys-LTs release by leucocytes from AIA and ATA, or by differentiated Hl-60 cells. Measurement of cysteinyl-leukotriene release by peripheral blood leucocytes pre-treated with aspirin has no value for diagnosis of AIA.
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Authors | M Pierzchalska, L Mastalerz, M Sanak, M Zazula, A Szczeklik |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 30
Issue 12
Pg. 1785-91
(Dec 2000)
ISSN: 0954-7894 [Print] England |
PMID | 11122218
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Leukotrienes
- N-Formylmethionine Leucyl-Phenylalanine
- Aspirin
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Topics |
- Adult
- Aspirin
(adverse effects, pharmacology)
- Asthma
(blood, chemically induced)
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Female
- HL-60 Cells
(drug effects, metabolism)
- Humans
- Leukocytes
(drug effects, metabolism)
- Leukotrienes
(analysis)
- Male
- N-Formylmethionine Leucyl-Phenylalanine
(pharmacology)
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