CD4(+) T cells have been reported to suppress immunity against
cancer in certain animal models. In this study, we investigated the role of CD4(+) T cells in the anti-
tumor immune response when interleukin-12-producing
melanoma cells are inoculated in mice. We found that interleukin-12-transfected
B16 melanoma showed retarded
tumor growth in syngeneic mice; however, all the mice developed
tumors eventually. In vivo depletion of CD4(+) T cells led to complete regression of B16/
interleukin-12 tumors in 12 of 20 mice (60%). Immunohistochemical analyses revealed that a number of CD8(+) T cells accumulated in close proximity to the B16/
interleukin-12 tumors in the CD4(+) T cell-depleted mice, whereas CD8(+) T cells were only scarcely observed at the periphery of the
tumors in control immunocompetent mice. Furthermore, 10 of 20 mice treated with both B16/
interleukin-12 inoculation and CD4(+) T cell depletion exhibited
vitiligo-like coat color alteration. B16/
interleukin-12 tumors completely regressed in all the mice with
vitiligo. Histologic examination showed that CD8(+) lymphocytes accumulated around the hair bulbs of mice with
vitiligo, but not in those without
vitiligo. These results suggest that CD4(+) T cells have an inhibitory effect on
tumor rejection by suppressing cytotoxic CD8(+) T cells in this
melanoma loading model with local
interleukin-12 secretion. To investigate the mechanism of enhanced anti-
tumor effects by CD4(+) T cell depletion, we examined the T helper type 1/2
cytokine profile in the
tumor draining lymph nodes of B16/
interleukin-12-bearing mice with or without CD4(+) T cell depletion using the reverse transcription-polymerase chain reaction method. We found that CD4(+) T cell depletion eliminated T helper type 2 cells and resulted in a T helper type 1-dominant
cytokine profile in
tumor draining lymph nodes. We emphasize that this T helper type 1-dominant
cytokine profile may generate further activated CD8(+) T cells against
B16 melanoma cells, lead B16/
interleukin-12 to regress, and result in the destruction of the melanocytes in hair bulbs due to cross-antigenicity between both cell types. This mouse model not only demonstrates the depletion of CD4(+) T cells as a useful strategy for cancer gene
therapy with
interleukin-12 but also provides a model for human
melanoma-associated
vitiligo.J Invest Dermatol 115:1059-1064 2000