| Abstract | Thymidine phosphorylase (dThdPase) is an essential enzyme for activation of the oral cytostatic drug capecitabine and its intermediate metabolite, doxifluridine, to 5-fluorouracil in tumors. Methods to estimate dThdPase expression in tumor tissue might be useful to predict the efficacy of capecitabine and doxifluridine in cancer patients. We established a new monoclonal antibody (MAb), 1C6-203, applicable for dThdPase immunohistochemistry and compared its staining characteristics with those of a previously established MAb, 654-1. In 4% paraformaldehyde-fixed colorectal carcinoma, 1C6-203 and 654-1 stained cancer cells in 19/30 and 9/30 patients, respectively. In 10% formalin-fixed colorectal carcinoma, 1C6-203 and 654-1 stained cancer cells in 18/30 and 6/30 patients, respectively. In negative 10% formalin-fixed tissues, microwave treatment improved the positivity of 654-1-stained cancer cells. These results suggest that an epitope recognized by 1C6-203 is resistant to epitope masking by formaldehyde fixation, whereas that for MAb 654-1 is sensitive. Therefore, MAb 1C6-203 might be more suitable than MAb 654-1 for evaluating dThdPase expression in colorectal carcinoma. |
| Authors | T Kono, M Nishida, N Inagaki, Y Tanaka, M Yoneda, S Kasai
(Affiliation: Second Department of Surgery and Medicine, Kamakura, Kanagawa, Japan. kono at asahikawa-med.ac.jp)
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| Journal | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
(J Histochem Cytochem)
Vol. 49
Issue 1
Pg. 131-8
(Jan 2001)
ISSN: 0022-1554 UNITED STATES |
| PMID | 11118485
(Publication Type: Comparative Study, Journal Article)
|
| Chemical References |
- Antibodies, Monoclonal
- Fixatives
- Polymers
- paraform
- Formaldehyde
- Thymidine Phosphorylase
|
| Topics |
- Animals
- Antibodies, Monoclonal
(biosynthesis)
- Antibody Specificity
- Blotting, Western
- Breast Neoplasms
(enzymology)
- Colorectal Neoplasms
(enzymology)
- Female
- Fixatives
- Formaldehyde
- Humans
- Immunohistochemistry
- Mice
- Mice, Inbred BALB C
- Polymers
- Stomach Neoplasms
(enzymology)
- Thymidine Phosphorylase
(immunology, metabolism)
- Tissue Fixation
|
