Sensitivity to interferon-alpha and interferon-stimulated gene factor 3 binding activity in human chronic myelogenous leukemia cell line KT-1.

Abstract

The mechanism of responsiveness of chronic myelogenous leukemia (CML) cells to interferon (IFN)-alpha was examined by using two subclones of CML cell line KT-1 which exhibited significantly different sensitivities to the antiproliferative and apoptosis-inducing effects of IFN-alpha. IFN-stimulated gene factor 3 (ISGF3) formation by IFN-alpha was reduced in the IFN-alpha-resistant subclone compared to the IFN-alpha-sensitive subclone. We conclude that the level of ISGF3 formation is responsible for the difference in IFN-alpha responses between these subclones.

• Authors: H Yamauchi, I Sakai, H Narumi, S Soga, S Fujita
• Journal: Acta haematologica (Acta Haematol) Vol. 104 Issue 1 Pg. 1-9 ( 2000) ISSN: 0001-5792 [Print] Switzerland
• PMID: 11111114 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright 2000 S. Karger AG, Basel

Chemical References

• Antineoplastic Agents
• DNA-Binding Proteins
• IRF9 protein, human
• Interferon-Stimulated Gene Factor 3
• Interferon-Stimulated Gene Factor 3, gamma Subunit
• Interferon-alpha
• Protein Subunits
• RNA, Messenger
• STAT1 Transcription Factor
• STAT1 protein, human
• STAT2 Transcription Factor
• Trans-Activators
• Transcription Factors
• Protein-Tyrosine Kinases

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Division (drug effects)
• Clone Cells (drug effects)
• DNA Fragmentation
• DNA-Binding Proteins (drug effects, genetics, metabolism, pharmacology, physiology)
• Electrophoresis, Agar Gel
• Flow Cytometry
• Humans
• Interferon-Stimulated Gene Factor 3
• Interferon-Stimulated Gene Factor 3, gamma Subunit
• Interferon-alpha (genetics, pharmacology, physiology)
• Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology)
• Promoter Regions, Genetic (drug effects)
• Protein Binding (physiology)
• Protein Subunits
• Protein-Tyrosine Kinases (physiology)
• RNA, Messenger (biosynthesis, drug effects)
• Response Elements (drug effects)
• STAT1 Transcription Factor
• STAT2 Transcription Factor
• Signal Transduction (drug effects)
• Time Factors
• Trans-Activators (drug effects, metabolism, physiology)
• Transcription Factors (drug effects, genetics, metabolism, pharmacology)
• Tumor Cells, Cultured (drug effects)