Adenosine A1 receptor blockade reverses dysmotility induced by ischemia-reperfusion in rat colon.

Abstract

This study was designed to assess whether adenosine A1 receptor antagonists [(R)-1-[(E)-3-(2-phenylpyrazolo[1,5-a]pyridin-3-yl) acryloyl]-piperidin-2-yl acetic acid (FK352) and 8-cyclopentyl-1,3-dipropylxanthine (DPCPX)] reverse dysmotility induced by ischemia-reperfusion in the rat colon. The gene of adenosine A1 receptor was expressed in the colon. Clamping (30 min) of the colonic marginal vessels was followed by reperfusion, and the propulsive colonic motility was evaluated. Propulsion was significantly slowed by ischemia-reperfusion, while FK352 and DPCPX abolished this delay. In contrast, the non-selective adenosine receptor antagonist, 8-phenyltheophylline, failed to affect the dysmotility. Thus, adenosine A1 receptor antagonists have potent therapeutic potential against ischemia-reperfusion-induced dysmotility in the colon.

• Authors: M Kadowaki, K Tokita, Y Nagakura, M Takeda, K Hanaoka, M Tomoi
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 409 Issue 3 Pg. 319-23 (Dec 15 2000) ISSN: 0014-2999 [Print] Netherlands
• PMID: 11108827 (Publication Type: Journal Article)

Chemical References

• FK 352
• Purinergic P1 Receptor Antagonists
• Pyrazoles
• Pyridines
• Receptors, Purinergic P1
• Xanthines
• 1,3-dipropyl-8-cyclopentylxanthine

Topics

• Animals
• Colon (drug effects, physiology)
• Gastrointestinal Motility (drug effects, physiology)
• Male
• Purinergic P1 Receptor Antagonists
• Pyrazoles (pharmacology, therapeutic use)
• Pyridines (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Receptors, Purinergic P1 (physiology)
• Reperfusion Injury (drug therapy)
• Xanthines (pharmacology, therapeutic use)