Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry.

Abstract

The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the staphylococcal surface using flow cytometry. Our results are in agreement with previous reports on the transcription of the respective genes and demonstrate different patterns of temporal expression for the two adhesins in the strains studied. We demonstrate a convenient technique for quantification of bacterial adhesins that can be used in studies aimed at characterization of bacterial adhesion to ECM components and understanding expression of adhesins during the course of an infection.

• Authors: N Mohamed, L Visai, P Speziale, J M Ross
• Journal: Microbial pathogenesis (Microb Pathog) Vol. 29 Issue 6 Pg. 357-61 (Dec 2000) ISSN: 0882-4010 [Print] England
• PMID: 11095920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Adhesins, Bacterial
• Antibodies, Monoclonal
• Bacterial Proteins
• Carrier Proteins
• Integrins
• Receptors, Collagen
• fibronectin-binding proteins, bacterial

Topics

• Adhesins, Bacterial (analysis, genetics, physiology)
• Antibodies, Monoclonal
• Bacterial Proteins (analysis, genetics, physiology)
• Carrier Proteins (analysis, genetics, physiology)
• Flow Cytometry
• Gene Expression Regulation, Bacterial
• Integrins (analysis, genetics, physiology)
• Receptors, Collagen
• Staphylococcus aureus (chemistry, genetics, physiology)
• Time Factors