The possible protective effect of
kolaviron on rat erythrocytes following simultaneous administration of
kolaviron (100 mg/kg of
body weight/day) with
carbon tetrachloride CCl4 (1.195 g/kg of
body weight/day) by separate
intraperitoneal injections was investigated.
Kolaviron, a
biflavonoid fraction of the defatted alcoholic extract of Garcinia kola seed, inhibits the accumulation of lipid peroxidation products in erythrocytes. A significant reduction (p < 0.05) by about 34%, of lipid peroxidation products was observed in erythrocytes of rats treated simultaneously with CCl4 and
kolaviron when compared to CCl4-treated rats. Similarly, the significant increase (p < 0.05) in membrane
cholesterol observed in CCl4-treated rats was significantly decreased (p < 0.05) in rats treated simultaneously with CCl4 and
kolaviron. Therefore, there was no significant difference (p < 0.05) in
cholesterol phospholipid ratio (C/P) of rats treated simultaneously with CCl4 and
kolaviron, and the controls. Thus,
kolaviron normalizes the CCl4-induced change in erythrocyte membrane composition. In addition,
kolaviron antagonizes the effect of CCl4 on the activity of the membrane bound
enzyme, Ca2+-
ATPase. These results suggest that
kolaviron protects erythrocyte membranes from
free radical attack, on both
lipids and
proteins.