Abstract |
In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/ endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.
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Authors | K Saida, T Uchide, A Usui, X D Gao, N Tomizuka, S Oka, H Masuda |
Journal | Journal of cardiovascular pharmacology
(J Cardiovasc Pharmacol)
Vol. 36
Issue 5 Suppl 1
Pg. S9-11
(Nov 2000)
ISSN: 0160-2446 [Print] United States |
PMID | 11078321
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endothelin-2
- Intercellular Signaling Peptides and Proteins
- Peptides
- RNA, Messenger
- vasoactive intestinal constrictor
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Topics |
- Animals
- Endothelin-2
(genetics)
- Genes, Reporter
- Intercellular Signaling Peptides and Proteins
- PC12 Cells
- Peptides
(genetics)
- Promoter Regions, Genetic
- RNA, Messenger
(analysis)
- Rats
- Reverse Transcriptase Polymerase Chain Reaction
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