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The endothelin-2/vasoactive intestinal contractor gene: expression and promoter activity in PC12 rat pheochromocytoma cells.

Abstract
In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.
AuthorsK Saida, T Uchide, A Usui, X D Gao, N Tomizuka, S Oka, H Masuda
JournalJournal of cardiovascular pharmacology (J Cardiovasc Pharmacol) Vol. 36 Issue 5 Suppl 1 Pg. S9-11 (Nov 2000) ISSN: 0160-2446 [Print] United States
PMID11078321 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Endothelin-2
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • RNA, Messenger
  • vasoactive intestinal constrictor
Topics
  • Animals
  • Endothelin-2 (genetics)
  • Genes, Reporter
  • Intercellular Signaling Peptides and Proteins
  • PC12 Cells
  • Peptides (genetics)
  • Promoter Regions, Genetic
  • RNA, Messenger (analysis)
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction

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