Abstract | BACKGROUND AND AIMS: METHODS: RESULTS: Normal colonic mucosa did not express gastrin or CCKBR. Gastrin and CCKBR reverse transcription-polymerase chain reaction products were detected and verified by specific hybridisation with an oligo probe on Southern blots. Gastrin and CCKBR were expressed in 78% and 81% of polyps, respectively. Both genes were coexpressed in 97% of cases. Immunohistochemistry identified progastrin in 91%, glycine extended gastrin 17 in 80%, and amidated gastrin 17 in only 47% of polyps. CCKBR was present in 96% of polyps. Expression of gastrin and CCKBR was seen in all histological types and sizes of polyps. CONCLUSIONS:
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Authors | A M Smith, S A Watson |
Journal | Gut
(Gut)
Vol. 47
Issue 6
Pg. 820-4
(Dec 2000)
ISSN: 0017-5749 [Print] England |
PMID | 11076881
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Gastrins
- Neoplasm Proteins
- Receptor, Cholecystokinin B
- Receptors, Cholecystokinin
|
Topics |
- Adenoma
(metabolism)
- Aged
- Carcinoma
(metabolism)
- Colonic Neoplasms
(metabolism)
- Colonic Polyps
(metabolism)
- Disease Progression
- Female
- Gastrins
(metabolism)
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neoplasm Proteins
(metabolism)
- Precancerous Conditions
(metabolism)
- Receptor, Cholecystokinin B
- Receptors, Cholecystokinin
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
|