Rat model of experimentally induced abacterial prostatitis.

Abstract

BACKGROUND:

An experimental model in rats was developed to investigate the significance of mucosal integrity in abacterial prostatitis.

METHODS:

Ethanol was instilled into the ventral prostates of male rats to reduce mucosal integrity; dinitrobenzenesulfonic acid (DNBS) was added as an irritant to induce inflammation. Controls received no treatment, ethanol only, DNBS only, or a suspension of bacteria. After various time points, rats were sacrificed, and their prostates were assayed for gross morphology, histological appearance, and cytokine levels.

RESULTS:

Prostates subjected to ethanol plus DNBS showed significant inflammation, most notably after 12, 24, and 48 hr. Inflammation judged by gross and histological observations and interleukin-1beta levels correlated well at these times. Rats given only ethanol, DNBS, or no treatment, acting as negative controls, displayed little or no inflammation; rats given a bacterial suspension, acting as positive controls, showed inflammation consistent with past studies. Cytokine assays revealed raised interleukin-1beta levels in this model, while tumor necrosis factor-alpha remained at a basal level.

CONCLUSIONS:

The loss of an intact mucosal surface in the prostate resulted in inflammation caused by an irritant. Interleukin-1beta appears to play a role in this inflammation, while tumor necrosis factor-alpha does not.

• Authors: M D Lang, J C Nickel, M E Olson, S R Howard, H Ceri
• Journal: The Prostate (Prostate) Vol. 45 Issue 3 Pg. 201-6 (Nov 01 2000) ISSN: 0270-4137 [Print] United States
• PMID: 11074521 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 Wiley-Liss, Inc.

Chemical References

• Benzenesulfonates
• Central Nervous System Depressants
• dinitrobenzenesulfonic acid
• Ethanol

Topics

• Animals
• Benzenesulfonates (administration & dosage, adverse effects)
• Central Nervous System Depressants (administration & dosage, adverse effects)
• Disease Models, Animal
• Ethanol (administration & dosage, adverse effects)
• Inflammation
• Male
• Mucous Membrane (pathology)
• Prostatitis (chemically induced, physiopathology)
• Rats
• Rats, Sprague-Dawley