Effect of cobalt ion on radical intensity and cytotoxic activity of antioxidants.

Abstract	The effect of CoCl2 on the cytotoxic activity of various antioxidants against human oral tumor cell lines (HSC-2, HSG) and normal human gingival fibroblasts (HGF) was investigated. Noncytotoxic concentrations of CoCl2 significantly reduced the cytotoxic activity of sodium ascorbate, gallic acid, epigallocatechin gallate (EGCG), curcumin and dopamine, but not that of sodium 5,6-benzylidene-L-ascorbate (SBA) and benzaldehyde. Among these compounds, benzaldehyde showed the most prominent tumor-specific cytotoxic action. ESR spectroscopy showed that these antioxidants produced radicals under alkaline condition and that their radical intensity was transiently enhanced and finally disappeared by addition of CoCl2. Antioxidants which are sensitive to CoCl2 generally had higher cytotoxic activity and oxidation potential (measured by NO monitor) and addition of CoCl2 significantly reduced their oxidation potential. The present study suggests that cobalt ion stimulates the oxidation of antioxidants to their inactive products.
Authors	T Sakagami, K Satoh, M Ishihara, H Sakagami, F Takeda, M Kochi, M Takeda
Journal	Anticancer research (Anticancer Res) 2000 Sep-Oct Vol. 20 Issue 5A Pg. 3143-50 ISSN: 0250-7005 [Print] Greece
PMID	11062735 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antioxidants Benzaldehydes Benzylidene Compounds Cations, Monovalent Free Radical Scavengers Cobalt Gallic Acid Catechin epigallocatechin gallate cobaltous chloride Curcumin Ascorbic Acid zilascorb benzaldehyde Dopamine
Topics	Antioxidants (chemistry, metabolism, pharmacology) Ascorbic Acid (analogs & derivatives, chemistry, metabolism, pharmacology) Benzaldehydes (chemistry, metabolism, pharmacology) Benzylidene Compounds (chemistry, metabolism, pharmacology) Catechin (analogs & derivatives, chemistry, metabolism, pharmacology) Cations, Monovalent Cell Line Child Cobalt (metabolism, pharmacology) Curcumin (chemistry, metabolism, pharmacology) Dopamine (chemistry, metabolism, pharmacology) Female Free Radical Scavengers (chemistry, metabolism, pharmacology) Gallic Acid (chemistry, metabolism, pharmacology) Humans Mouth Neoplasms Oxidation-Reduction Tumor Cells, Cultured

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