Abstract |
Although human papillomaviruses (HPV) and adenoviruses (Ad) both transform cells by expressing functionally related oncogenes (Ad-E1A/E1B; HPV-E7/E6), only HPV are oncogenic in humans. Prior studies have shown that HPV-transformed cells are resistant to NK cell lysis and E7- and E6-specific CTL are inefficiently generated in women with HPV-induced cervical cancer. Therefore, we postulated that the dissimilar oncogenicities of Ad and HPV may be caused by a protective NK and T cell response that is triggered by transformed cells expressing E1A, but not by E7. To test this hypothesis, mice that were either immunologically intact, lacked T cells, or lacked both NK and T cells were challenged with Ad serotype 5 (Ad5)-E1A- or HPV16-E7-transfected tumor cells. E7-expressing tumor cells were resistant to NK cell lysis in vitro and failed to elicit a measurable anti- tumor NK or T cell response in vivo. The concomitant expression of E6 did not change this phenotype. In contrast, E1A-expressing tumor cells were sensitive to NK lysis in vitro and triggered a protective NK and T cell immune response in vivo. These data suggest differences in the capacities of E1A or E7 oncoproteins to trigger protective anti- tumor immune responses may contribute to the dissimilar oncogenicities of Ad and HPV in humans.
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Authors | J M Routes, S Ryan, H Li, J Steinke, J L Cook |
Journal | Virology
(Virology)
Vol. 277
Issue 1
Pg. 48-57
(Nov 10 2000)
ISSN: 0042-6822 [Print] United States |
PMID | 11062035
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2000 Academic Press. |
Chemical References |
- Adenovirus E1A Proteins
- Oncogene Proteins, Viral
- Papillomavirus E7 Proteins
- oncogene protein E7, Human papillomavirus type 16
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Topics |
- Adenovirus E1A Proteins
(immunology)
- Adenoviruses, Human
(genetics)
- Animals
- Cytotoxicity, Immunologic
- Female
- Fibrosarcoma
(immunology, virology)
- Humans
- Killer Cells, Natural
(immunology)
- Mice
- Mice, Inbred C57BL
- Mice, Nude
- Mice, Transgenic
- Oncogene Proteins, Viral
(immunology)
- Oncogenes
- Papillomaviridae
(genetics)
- Papillomavirus E7 Proteins
- Serotyping
- T-Lymphocytes
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
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