Abstract | PURPOSE: MATERIALS AND METHODS: A total of 174 patients comprised the study, including 80 who were evaluated because of hematuria or irritative voiding symptoms and 94 who were examined during followup visits after resection of bladder tumors. Voided urine samples were obtained for immunocytology and cytopathology. Each patient underwent cystoscopy, and biopsies were done when a bladder tumor was detected or carcinoma in situ was suspected. Indirect immunoperoxidase staining was done on cytocentrifuge slides using a monoclonal antibody against cytokeratin 20. RESULTS:
Bladder tumors were found in 87 patients. Immunocytology resulted in 81.6% sensitivity, 77% specificity and 80% accuracy. False-negative results occurred in 12 patients with superficial low grade tumors and in 4 with high grade invasive tumors. Cytopathology resulted in 51.7% sensitivity and 94.2% specificity. CONCLUSIONS: Immunocytology of the cytokeratin 20 antigen is significantly more sensitive than cytopathology for bladder tumor detection, especially low grade and low stage cancer. The results are not affected by inflammation or previous treatments with bacillus Calmette-Guerin. Immunocytology may miss high grade tumors and, therefore, cannot be used alone as a substitute for cystoscopy.
|
Authors | D Golijanin, A Shapiro, D Pode |
Journal | The Journal of urology
(J Urol)
Vol. 164
Issue 6
Pg. 1922-5
(Dec 2000)
ISSN: 0022-5347 [Print] United States |
PMID | 11061882
(Publication Type: Journal Article)
|
Chemical References |
- Biomarkers, Tumor
- Intermediate Filament Proteins
- KRT20 protein, human
- Keratin-20
|
Topics |
- Biomarkers, Tumor
(urine)
- Female
- Humans
- Immunohistochemistry
- Intermediate Filament Proteins
(analysis)
- Keratin-20
- Male
- Predictive Value of Tests
- Sensitivity and Specificity
- Urinary Bladder
(pathology)
- Urinary Bladder Neoplasms
(diagnosis, pathology, urine)
- Urine
(cytology)
- Urothelium
(chemistry)
|