Abstract |
Several alpha(1,3/1,4) fucosyltransferases expressed in human pancreatic cancer cells can participate in the biosynthesis of cell surface sialyl-Lewis a and sialyl-Lewis x antigens that contribute to hematogenous metastatis. Previously, we observed a significant increase of the alpha(1,4) fucosyltransferase activity in tumoral pancreatic cell lines, suggesting that FUT3 could be involved in the sialyl-Lewis antigen expression. Therefore, we invalidated the expression of FUT3 by expressing FUT3 antisense sequence in the human pancreatic tumor BxPC-3 cell line, which expresses the alpha(1,4) fucosyltransferase activity and harbors the cell surface sialyl-Lewis antigens. The decrease of FUT3 transcript after transfection of antisense cDNA of FUT3 in these cells results in a substantial reduction of sialyl-Lewis antigen expression on cell surface. This decreased antigen expression was associated with an inhibition of adhesive properties to E-selectin and a decrease of metastatic power of FUT3 antisense-transfected BxPC-3 cells as tested in nude mice. Our study provides evidence that the expression level of FUT3 may regulate the expression of sialyl-Lewis a and sialyl-Lewis x surface antigens and consequently could play an important role in metastatic properties of human pancreatic cancer cells.
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Authors | M Aubert, L Panicot-Dubois, C Crotte, V Sbarra, D Lombardo, M O Sadoulet, E Mas |
Journal | International journal of cancer
(Int J Cancer)
Vol. 88
Issue 4
Pg. 558-65
(Nov 15 2000)
ISSN: 0020-7136 [Print] United States |
PMID | 11058871
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2000 Wiley-Liss, Inc. |
Chemical References |
- E-Selectin
- Oligosaccharides
- RNA, Antisense
- RNA, Messenger
- Sialyl Lewis X Antigen
- Fucosyltransferases
- 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
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Topics |
- Animals
- CHO Cells
- Cricetinae
- E-Selectin
(genetics, physiology)
- Female
- Fucosyltransferases
(genetics, metabolism)
- Humans
- Mice
- Mice, Nude
- Oligosaccharides
(analysis)
- Pancreatic Neoplasms
(enzymology, genetics, pathology)
- Peritoneal Neoplasms
(genetics, pathology, prevention & control, secondary)
- RNA, Antisense
(genetics)
- RNA, Messenger
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Sialyl Lewis X Antigen
- Transcription, Genetic
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
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