Usefulness of (99m)Tc-d,l-HMPAO for estimation of GSH content in tumor tissues.

Abstract

To investigate whether [(99m)Tc]-hexamethyl propyleneamine oxime ([(99m)Tc]-HMPAO) is applicable for evaluating glutathione (GSH) localization in tumor, the difference of distribution between [(99m)Tc]-d,l- and meso-HMPAO was studied using a mouse tumor model. Biodistribution of [(99m)Tc]-d,l- or meso-HMPAO was studied in GSH-depleted and control Ehrlich tumor-bearing mice. GSH levels in tumors in GSH-depleted and control mice were measured in another set of mice. The uptake of [(99m)Tc]-d,l-HMPAO in tumor was significantly decreased by the diethyl maleate (DEM) treatment. On the other hand, the DEM treatment increased the accumulation of [(99m)Tc]-meso-HMPAO in tumor. Meanwhile, the content of GSH was lowest in tumor among the tissues tested and decreased in a manner similar to other tissues on preloading of DEM. [(99m)Tc]-d,l-HMPAO may be useful for estimating the GSH status in a certain tumor and thereby contribute to the diagnosis of anticancer therapy.

• Authors: N Shimura, A Musya, T Hashimoto, S Kojima, A Kubodera, T Sasaki
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 27 Issue 6 Pg. 577-80 (Aug 2000) ISSN: 0969-8051 [Print] United States
• PMID: 11056372 (Publication Type: Journal Article)

Chemical References

• Maleates
• Technetium Tc 99m Exametazime
• diethyl maleate
• Glutathione

Topics

• Animals
• Brain (diagnostic imaging, metabolism)
• Carcinoma, Ehrlich Tumor (diagnostic imaging, metabolism)
• Glutathione (metabolism)
• Kidney (diagnostic imaging, metabolism)
• Liver (diagnostic imaging, metabolism)
• Lung (diagnostic imaging, metabolism)
• Male
• Maleates (pharmacology)
• Mice
• Mice, Inbred Strains
• Neoplasm Transplantation
• Organ Specificity (drug effects)
• Radionuclide Imaging
• Spleen (diagnostic imaging, metabolism)
• Stereoisomerism
• Technetium Tc 99m Exametazime (metabolism, pharmacokinetics)
• Tissue Distribution