Abstract | BACKGROUND & AIMS: METHODS: A recombinant fusion protein, consisting of the extracellular portion of mouse TbetaR-II and the Fc portion of mouse immunoglobulin (Ig) G, was produced. A 5-cm segment of mouse ileum was exposed to 19 Gy x-radiation. TbetaR-II:Fc fusion protein (1 mg/kg every other day) or mouse IgG was administered from 2 days before to 6 weeks after irradiation. Radiation injury was assessed at 6 weeks using quantitative histology, morphometry, and immunohistochemistry. Collagen was measured colorimetrically, and TGF-beta1 messenger RNA was assessed with fluorogenic probe reverse-transcription polymerase chain reaction. RESULTS: Compared with IgG controls, TbetaR-II:Fc-treated mice exhibited less structural injury, preservation of mucosal surface area, and less intestinal wall fibrosis. Intestinal TGF-beta1 messenger RNA increased in TbetaR-II:Fc-treated mice, whereas TGF-beta immunoreactivity decreased. TbetaR-II:Fc treatment increased crypt cell proliferation but otherwise did not affect unirradiated intestine. CONCLUSIONS:
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Authors | H Zheng, J Wang, V E Koteliansky, P J Gotwals, M Hauer-Jensen |
Journal | Gastroenterology
(Gastroenterology)
Vol. 119
Issue 5
Pg. 1286-96
(Nov 2000)
ISSN: 0016-5085 [Print] United States |
PMID | 11054386
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Immunoglobulin Fc Fragments
- Immunoglobulin G
- Protein Isoforms
- RNA, Messenger
- Receptors, Transforming Growth Factor beta
- Recombinant Fusion Proteins
- Tgfb1 protein, mouse
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
- Collagen
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Topics |
- Animals
- CHO Cells
- Collagen
(metabolism)
- Cricetinae
- Ileum
(drug effects, metabolism, pathology, radiation effects)
- Immunoglobulin Fc Fragments
(genetics)
- Immunoglobulin G
(genetics)
- Intestinal Diseases
(drug therapy, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Protein Isoforms
(chemistry, genetics)
- RNA, Messenger
(metabolism)
- Radiation Injuries
(drug therapy, pathology)
- Receptors, Transforming Growth Factor beta
(chemistry, genetics)
- Recombinant Fusion Proteins
(therapeutic use)
- Solubility
- Transforming Growth Factor beta
(genetics, metabolism)
- Transforming Growth Factor beta1
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