Expression of pituitary homeo box 1 (Ptx1) in human non-neoplastic pituitaries and pituitary adenomas.

Abstract

We investigated the localization of pituitary homeo box 1 (Ptx1) protein in five human non-neoplastic pituitaries and 73 of all types of pituitary adenomas using immunohistochemistry, and the expression of Ptx1 messenger RNA (mRNA) in 18 representative pituitary adenomas using the reverse transcriptase polymerase chain reaction (RT-PCR) technique. By immunohistochemical analysis, Ptx1 protein was extensively detected in the nuclei of normal human pituitary cells. Ptx1 was detected in 10/14 (71.4%) of growth hormone (GH)-secreting adenomas, 12/12 (100%) of prolactin (PRL)-secreting adenomas, 18/20 (90%) of adrenocorticotropic hormone (ACTH)-secreting adenomas, 6/7 (85.7%) of thyroid-stimulating hormone (TSH)-secreting adenomas, and 17/20 (85%) of clinically non-functioning adenomas, including 9/10 (90%) of gonadotropin-subunit-positive adenomas. Thus, there was no relationship between Ptx1 expression and a particular type of pituitary adenomas. By RT-PCR analysis, Ptx1 mRNA was expressed in all 18 cases of pituitary adenomas, including two cases negative for Ptx1 protein by immunohistochemistry. These results suggested that Ptx1 may be an universal transcription factor in both neoplastic and non-neoplastic conditions in human pituitaries. The synergistic action with other transcription factors may be speculated to determine the specific production of the anterior pituitary hormones.

• Authors: S Tahara, R Kurotani, N Sanno, I Takumi, S Yoshimura, R Y Osamura, A Teramoto
• Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (Mod Pathol) Vol. 13 Issue 10 Pg. 1097-108 (Oct 2000) ISSN: 0893-3952 [Print] United States
• PMID: 11048804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Homeodomain Proteins
• Paired Box Transcription Factors
• Pituitary Hormones
• RNA, Messenger
• RNA, Neoplasm
• Transcription Factors
• homeobox protein PITX1

Topics

• Adenoma (metabolism, pathology)
• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Blotting, Western
• DNA Primers (chemistry)
• Female
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Paired Box Transcription Factors
• Pituitary Gland (metabolism, pathology)
• Pituitary Hormones (metabolism)
• Pituitary Neoplasms (metabolism, pathology)
• RNA, Messenger (metabolism)
• RNA, Neoplasm (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription Factors (genetics, metabolism)