Abstract | OBJECTIVE: METHODS:
AECA in sera of 15 SLE patients were measured by ELISA and Western blot analysis was used to examine the diversity of autoantigen targets in two clinically active patients. A human umbilical vein endothelial cell cDNA expression library was immunoscreened with sera from these two patients to identify their autoantigen targets. An anti-ribosomal P peptide antibody ELISA was used to assess the clinical significance of anti-ribosomal P protein antibodies in the sera of one patient. RESULTS: CONCLUSIONS: A panel of candidate endothelial autoantigens in SLE, which includes previously described autoantigens and novel targets, has been identified by a molecular cloning strategy. This novel molecular approach could also be applied to the identification of autoantigens in other autoimmune vascular diseases.
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Authors | G Frampton, S Moriya, J D Pearson, D A Isenberg, F J Ward, T A Smith, A Panayiotou, N A Staines, J J Murphy |
Journal | Rheumatology (Oxford, England)
(Rheumatology (Oxford))
Vol. 39
Issue 10
Pg. 1114-20
(Oct 2000)
ISSN: 1462-0324 [Print] England |
PMID | 11035132
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Autoantigens
- Phosphoproteins
- Ribosomal Proteins
- ribosomal protein P0
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Topics |
- Autoantibodies
(analysis)
- Autoantigens
(analysis)
- Blotting, Western
- Cloning, Molecular
- Endothelium, Vascular
(immunology, pathology)
- Gene Library
- Humans
- Longitudinal Studies
- Lupus Erythematosus, Systemic
(blood, immunology)
- Lupus Nephritis
(immunology)
- Phosphoproteins
(immunology)
- Ribosomal Proteins
(immunology)
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