Correlated gene expression between beta-1,4-galactosyltransferase V and N-acetylglucosaminyltransferase V in human cancer cell lines.

Abstract

Since our previous study showed that the gene expression level of beta-1,4-galactosyltransferase (beta-1,4-GalT) V is only increased in mouse NIH3T3 transformant and that beta-1,4-GalT V preferentially galactosylates the GlcNAcbeta1 --> 6Man branch of oligosaccharides [Shirane et al. (1999) Biochem. Biophys. Res. Commun. 265, 434-438], whether its gene expression is correlated with malignant transformation was investigated. Northern blot analysis of beta-1, 4-GalTs I, II, III, IV, V, and VI and N-acetylglucosaminyltransferase (GlcNAcT)V in human cancer cell lines showed that the gene expression levels of beta-1,4-GalT V but not other beta-1,4-GalTs are strongly correlated with those of GlcNAcT V whose activity was shown to increase by malignant transformation. These results indicate that beta-1,4-GalT V is involved in the galactosylation of highly branched oligosaccharides characteristic of malignantly transformed cells.

• Authors: T Sato, K Shirane, M Kido, K Furukawa
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 276 Issue 3 Pg. 1019-23 (Oct 05 2000) ISSN: 0006-291X [Print] United States
• PMID: 11027584 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• RNA, Messenger
• N-Acetylglucosaminyltransferases
• N-Acetyllactosamine Synthase

Topics

• Blotting, Northern
• Cell Transformation, Neoplastic (genetics, metabolism)
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Glycosylation
• Humans
• N-Acetylglucosaminyltransferases (genetics)
• N-Acetyllactosamine Synthase (genetics)
• RNA, Messenger (analysis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured