Abstract | PURPOSE: MATERIALS AND METHODS: Applying coagulation current to the male rat urethra produced urethral strictures. Halofuginone was given to the animals for 7 days, starting on the day of stricture formation, either orally at 1 and 5 ppm in the diet or by injection of 0.03% halofuginone solution into the urethra. All rats were sacrificed on day 21. Collagen alpha1(I) gene expression was evaluated by in situ hybridization, collagen content by sirius red staining and urethral morphology by urethrogram. RESULTS: Coagulation current produced reproducible strictures with a typical urethrogram appearance, which were associated with increases in collagen alpha1(I) gene expression and collagen content at the stricture site. Halofuginone injected into the urethra or orally at 5 ppm normalized the urethrogram and prevented increases in collagen alpha1(I) gene expression and collagen content. Halofuginone at a concentration of 10-8 M. inhibited the collagen secreted by fibroblasts derived from the rat male urethra, which was due to inhibition of the collagen alpha1(I) gene expression. CONCLUSIONS:
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Authors | A Nagler, O Gofrit, M Ohana, D Pode, O Genina, M Pines |
Journal | The Journal of urology
(J Urol)
Vol. 164
Issue 5
Pg. 1776-80
(Nov 2000)
ISSN: 0022-5347 [Print] United States |
PMID | 11025768
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Piperidines
- Protein Synthesis Inhibitors
- Quinazolines
- Quinazolinones
- Collagen
- halofuginone
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Topics |
- Animals
- Collagen
(biosynthesis)
- Disease Models, Animal
- Immunohistochemistry
- In Situ Hybridization
- Male
- Piperidines
- Protein Synthesis Inhibitors
(therapeutic use)
- Quinazolines
(therapeutic use)
- Quinazolinones
- Rats
- Rats, Sprague-Dawley
- Urethral Stricture
(prevention & control)
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