Diaspirin cross-linked hemoglobin improves oxygen extraction capabilities in endotoxic shock.

Abstract

We studied the effects of diaspirin cross-linked hemoglobin (DCLHb), a cell-free hemoglobin derived from human erythrocytes, on blood flow distribution and tissue oxygen extraction capabilities in endotoxic shock. Eighteen pentobarbital sodium-anesthetized, mechanically ventilated dogs received 2 mg/kg of E. coli endotoxin, followed by saline resuscitation to restore cardiac filling pressures to baseline levels. The animals were randomly divided into three groups: six served as control, six received DCLHb at a dose of 500 mg/kg (group 1) and six DCLHb at a dose of 1,000 mg/kg (group 2). Cardiac tamponade was then induced by saline injection in the pericardial sac to progressively reduce cardiac index and thereby allow study of tissue oxygen extraction capabilities. DCLHb had a dose-dependent vasopressor effect but did not significantly alter cardiac index or regional blood flow. During cardiac tamponade, critical oxygen delivery was 12.8 +/- 0.7 ml. kg(-1). min(-1) in the control group, but 8.6 +/- 0.9 and 8.2 +/- 0.7 ml. kg(-1). min(-1) in groups 1 and 2, respectively (both P < 0.05 vs. control group). The critical oxygen extraction ratio was 39.1 +/- 3.1% in the control group but 58.7 +/- 12.8% and 60.2 +/- 9.0% in groups 1 and 2, respectively. We conclude that DCLHb can improve whole body oxygen extraction capabilities during endotoxic shock in dogs.

• Authors: J Creteur, H Zhang, D De Backer, Q Sun, J L Vincent
• Journal: Journal of applied physiology (Bethesda, Md. : 1985) (J Appl Physiol (1985)) Vol. 89 Issue 4 Pg. 1437-44 (Oct 2000) ISSN: 8750-7587 [Print] United States
• PMID: 11007580 (Publication Type: Journal Article)

Chemical References

• Blood Substitutes
• Endotoxins
• Hemoglobins
• diaspirin-cross-linked hemoglobin
• Aspirin
• Oxygen

Topics

• Animals
• Aspirin (analogs & derivatives, pharmacology)
• Blood Pressure (drug effects)
• Blood Substitutes (pharmacology)
• Cardiac Output (drug effects)
• Cardiac Tamponade
• Dogs
• Endotoxins
• Escherichia coli
• Hemodynamics (drug effects, physiology)
• Hemoglobins (pharmacology)
• Oxygen (blood)
• Oxygen Consumption (drug effects)
• Regional Blood Flow (drug effects)
• Shock, Septic (blood, physiopathology, therapy)