HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Differential expression of proteoglycans biglycan and decorin during neointima formation after stent implantation in normal and atherosclerotic rabbit aortas.

Abstract
Proteoglycans decorin and biglycan, which bind to TGF-beta, are thought to participate in regulation of extracellular matrix accumulation in arterial intimal hyperplasia. To investigate the correlation of these proteoglycans with the cellular localization and phenotypic modulation of smooth muscle cells (SMCs), we analyzed the spatial and chronological distribution of these proteoglycans and two cytokines, TGF-beta and IL-1beta, in the process of neointima formation after stent implantation in the aortas of rabbits fed a high-cholesterol diet (atherosclerotic group) or a regular diet (control group). We implanted metallic stents in the rabbit aortas and harvested the aortas 4-56 days later for immunohistochemical and mRNA in situ hybridization analyses. In the control group, TGF-beta and biglycan expression was in correspondence with the chronology and localization of embryonic SMCs. In the atherosclerotic group, TGF-beta and biglycan expression was sustained throughout the experimental period, which was in accord with the prolonged expression of embryonic SMCs. Decorin, which did not occur in neointima in the control group, appeared in the atherosclerotic aortas in the confined area of vascular SMCs surrounding the macrophages around the stent wire. These results indicate that biglycan and decorin kinetics during neointima formation after arterial injury are distinct, despite their similar construction; biglycan synthesis correlates with embryonic SMCs.
AuthorsT Yamakawa, H Z Bai, J Masuda, Y Sawa, R Shirakura, J Ogata, H Matsuda
JournalAtherosclerosis (Atherosclerosis) Vol. 152 Issue 2 Pg. 287-97 (Oct 2000) ISSN: 0021-9150 [Print] Ireland
PMID10998456 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biglycan
  • Decorin
  • Extracellular Matrix Proteins
  • Interleukin-1
  • Proteoglycans
  • RNA, Messenger
  • Transforming Growth Factor beta
Topics
  • Animals
  • Aorta (metabolism, pathology, surgery)
  • Arteriosclerosis (metabolism, pathology, surgery)
  • Biglycan
  • Decorin
  • Extracellular Matrix (pathology)
  • Extracellular Matrix Proteins
  • Immunohistochemistry
  • In Situ Hybridization
  • Interleukin-1 (biosynthesis)
  • Male
  • Proteoglycans (biosynthesis)
  • RNA, Messenger (analysis)
  • Rabbits
  • Stents
  • Transforming Growth Factor beta (biosynthesis)
  • Tunica Intima (pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: