Chemopreventative activity of crude hydroxymatairesinol (HMR) extract in Apc(Min) mice [corrected].

Abstract

We studied the effects of a lignan, hydroxymatairesinol (HMR), and rye bran on intestinal tumor development in adenomatous polyposis colimultiple intestinal neoplasia (Apc)(Min) mice. HMR showed a strong chemopreventive effect in this animal model. The mean number of adenomas in the small intestine was significantly lower (26.6+/-11.0, P<0.05) in mice fed the TNS tumor promoter insulin and HMR when compared with the insulin and insulin/rye bran fed mice (39.6+/-8.9 and 36.0+/-7.4, respectively). HMR resulted in normalization of beta-catenin levels in adenoma tissue, indicating that HMR mediates its chemopreventive effect through the Apc-beta-catenin pathway. In the cytosolic fraction, beta-catenin level in adenoma tissue was significantly elevated (P=0.008-0.013) in all the diet groups as compared with that of the surrounding mucosa. In the nuclear fraction, beta-catenin in the insulin (3.15+/-2.9 relative units) and insulin/rye (5.17+/-6.94 relative units) groups was also significantly higher (P=0.003-0.009) in the adenoma tissue when compared with the surrounding mucosa (0.5+/-0.5 and 0.35+/-0.39 relative units). However, HMR was able to restore nuclear beta-catenin level of the adenoma tissue (0.41+/-0.25 relative units) to the level found in the surrounding mucosa (0.36+/-0.28 relative units).

• Authors: S I Oikannen, A M Pajari, M Mutanen
• Journal: Cancer letters (Cancer Lett) Vol. 159 Issue 2 Pg. 183-7 (Oct 31 2000) ISSN: 0304-3835 [Print] Ireland
• PMID: 10996730 (Publication Type: Journal Article)

Chemical References

• Anticarcinogenic Agents
• CTNNB1 protein, mouse
• Cytoskeletal Proteins
• Insulin
• Lignans
• Trans-Activators
• beta Catenin

Topics

• Adenomatous Polyposis Coli (genetics, metabolism, prevention & control)
• Animals
• Anticarcinogenic Agents (pharmacology)
• Cell Nucleus (drug effects, metabolism)
• Cytoskeletal Proteins (drug effects, metabolism)
• Cytosol (drug effects, metabolism)
• Insulin (pharmacology)
• Intestinal Neoplasms (genetics, metabolism, prevention & control)
• Intestine, Small (drug effects, metabolism, pathology)
• Lignans (pharmacology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Trans-Activators
• beta Catenin