Diarylheptanoids suppress expression of leukocyte adhesion molecules on human vascular endothelial cells.

Abstract

Diarylheptanoids possess potent anti-inflammatory properties. However, the mechanism of their action is not fully understood. In this study, we found that three diarylheptanoids, 1-(3, 5-dimethoxy-4-hydroxyphenyl)-7-phenylhept-1-en-3-one (YPE-01), yakuchinone B and demethyl-yakuchinone B, reduced the adhesion of both human monocytic cell line U937 and human eosinophilic cell line EoL-1 cells to tumor necrosis factor-alpha (TNF-alpha)-treated human umbilical vein endothelial cells. In addition, they suppressed interleukin-1beta- or TNF-alpha-induced expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on the surface of the endothelial cells. Since YPE-01 reduced both VCAM-1 and ICAM-1 mRNA induction in TNF-alpha-stimulated endothelial cells, diarylheptanoids appeared to suppress adhesion molecule expression at the transcriptional level. Furthermore, YPE-01 suppressed both VCAM-1 and ICAM-1 mRNA induction as well as edema in 12-O-tetradecanoylphorbol 13-acetate (TPA)-inflamed mice ears in vivo. These results suggest that the anti-inflammatory action of diarylheptanoids is, at least in part, due to their suppressive effect on the surface expression of inducible adhesion molecules in endothelial cells, and subsequent leukocyte adhesion.

• Authors: R Yamazaki, H Hatano, R Aiyama, T Matsuzaki, S Hashimoto, T Yokokura
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 404 Issue 3 Pg. 375-85 (Sep 22 2000) ISSN: 0014-2999 [Print] Netherlands
• PMID: 10996603 (Publication Type: Journal Article)

Chemical References

• Cell Adhesion Molecules
• Diarylheptanoids
• E-Selectin
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• yakuchinone B
• Intercellular Adhesion Molecule-1
• Guaiacol

Topics

• Animals
• Cell Adhesion Molecules (drug effects, metabolism)
• Cells, Cultured
• Diarylheptanoids
• E-Selectin (drug effects, metabolism)
• Edema (drug therapy, metabolism)
• Endothelium, Vascular (drug effects, metabolism)
• Guaiacol (analogs & derivatives, pharmacology, therapeutic use)
• Humans
• Intercellular Adhesion Molecule-1 (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Phytotherapy
• Plants, Medicinal (therapeutic use)
• Tumor Cells, Cultured
• Tumor Necrosis Factor-alpha (pharmacology)
• Umbilical Veins (drug effects, metabolism)
• Vascular Cell Adhesion Molecule-1 (drug effects, metabolism)