Abstract |
The hypothesis of the present work was that expression of matrix metalloproteinase-13 (MMP-13, collagenase-3) would be induced during conditions involving important matrix remodeling such as ligament maturation, scar healing and joint instability. Therefore, MMP-13 expression in the medial collateral ligament (MCL) during the variable situations of tissue maturation and healing was assessed. MMP-13 expression in three intra-articular connective tissues of the knee (i.e. articular cartilage, menisci and synovium) following the transection of the anterior cruciate ligament of the knee was evaluated at 3 and 8 weeks post-injury. MMP-13 mRNA (semi-quantitative RT-PCR) and protein (immunohistochemistry and Western blotting) were detected in all of the tissues studied. Significantly higher MCL mRNA levels for MMP-13 were detected during the early phases of tissue maturation (i.e. 29 days in utero and 2-month-old rabbits) compared to later phases (5- and 12-month-old rabbits). This pattern of expression was recapitulated following MCL injury, with very high levels of expression in scar tissue at 3 weeks post-injury and then a decline to levels not significantly different from control values by 14 weeks. Elevated mRNA levels correlated with increased protein levels for MMP-13 in both menisci and synovium following the transection of the anterior cruciate ligament and during medial collateral ligament healing. These results indicate that MMP-13 expression is regulated by a number of variables and that high levels of expression occur in situations when connective tissue remodeling is very active.
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Authors | M P Hellio Le Graverand, J Eggerer, P Sciore, C Reno, E Vignon, I Otterness, D A Hart |
Journal | Matrix biology : journal of the International Society for Matrix Biology
(Matrix Biol)
Vol. 19
Issue 5
Pg. 431-41
(Sep 2000)
ISSN: 0945-053X [Print] Netherlands |
PMID | 10980419
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- RNA, Messenger
- Collagen
- Collagenases
- Matrix Metalloproteinase 13
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Topics |
- Animals
- Anterior Cruciate Ligament Injuries
- Base Sequence
- Cartilage, Articular
(enzymology)
- Collagen
(metabolism)
- Collagenases
(genetics, metabolism)
- Connective Tissue
(enzymology, growth & development, injuries)
- DNA Primers
(genetics)
- Female
- Gene Expression
- Knee Injuries
(enzymology, genetics)
- Knee Joint
(enzymology, growth & development)
- Matrix Metalloproteinase 13
- Medial Collateral Ligament, Knee
(enzymology, growth & development, injuries)
- Menisci, Tibial
(enzymology)
- RNA, Messenger
(genetics, metabolism)
- Rabbits
- Synovial Membrane
(enzymology)
- Wound Healing
(genetics, physiology)
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