Human tumor necrosis factor-alpha mutant RGD-V29 (F4614) shows potent antitumor activity and reduced toxicity against human tumor xenografted nude mice.

Abstract

The antitumor effects of human tumor necrosis factor-alpha (TNF) mutant RGD-V29 (code no. F4614), that includes the cell adhesive sequence (4)Arg-(5)Gly-(6)Asp and (29)Arg-->Val substitution, were evaluated. The therapeutic index, a measure of the extent of the therapeutically-effective range, using three constitutive administrations of RGD-V29 in Meth A-bearing mice was 4.8, whereas that of recombinant human TNF (rhTNF) ((1)SSS(4)RTPSDK...(29)RR...(155)L) was 2.8, clearly indicating that the effective RGD-V29 dose-range was extended. Furthermore, RGD-V29 showed potent antitumor activity against human lung cancer Mqnu-1 xenografted nude mice without severe gastrointestinal and other organ toxicities, even when administered at the maximal tolerated dose (MTD). In contrast, rhTNF induced severe toxicity at the MTD. Direct cytotoxicity of RGD-V29 against Mqnu-1 cells was similar to that of rhTNF. In addition, a cytotoxicity assay using a tumor-derived endothelial-like cell (tEC)/normal endothelial cell (nEC) system used to study TNF antitumor effects on tumor-associated endothelial cells, suggested that RGD-V29 showed preferential cytotoxicity toward tumor-associated endothelial cells compared with rhTNF. Thus, RGD-V29 appears to be a low-toxicity mutant of rhTNF that shows preferential activity towards tumors, and therefore merits further investigation in pre-clinical and clinical studies.

• Authors: K Kuroda, K Miyata, F Fujita, M Koike, M Fujita, M Nomura, S Nakagawa, Y Tsutsumi, T Kawagoe, Y Mitsuishi, T Mayumi
• Journal: Cancer letters (Cancer Lett) Vol. 159 Issue 1 Pg. 33-41 (Oct 16 2000) ISSN: 0304-3835 [Print] Ireland
• PMID: 10974403 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antineoplastic Agents
• F 4614
• Recombinant Proteins
• Tumor Necrosis Factor-alpha

Topics

• Amino Acid Sequence
• Amino Acid Substitution
• Animals
• Antineoplastic Agents (pharmacology, therapeutic use)
• Cell Line
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Humans
• Lung Neoplasms (drug therapy, pathology)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Recombinant Proteins (pharmacology, therapeutic use)
• Time Factors
• Transplantation, Heterologous
• Tumor Cells, Cultured
• Tumor Necrosis Factor-alpha (genetics, pharmacology, therapeutic use)