Stimulatory effects of dibutyryl cyclic adenosine monophosphate on cytokine production by keratinocytes and fibroblasts.

Abstract

BACKGROUND:

Clinical studies have shown that ointment containing dibutyryl cyclic adenosine monophosphate (DBcAMP) promotes wound healing.

OBJECTIVES:

We aimed to elucidate the mechanisms of the beneficial effect of DBcAMP in wound healing.

METHODS:

An investigation was made of the effects of DBcAMP on in vitro cytokine release from cultured keratinocytes and fibroblasts derived from normal human skin.

RESULTS:

DBcAMP stimulated keratinocyte proliferation through increased interleukin (IL)-6 production by fibroblasts, and transiently enhanced production of transforming growth factor (TGF)-beta1 by fibroblasts at an early stage of incubation. DBcAMP also stimulated fibroblast proliferation, resulting in further increases in IL-6 and TGF-beta1.

CONCLUSIONS:

We conclude that this series of stimulative actions on cytokine secretion, together with the facilitation of cell proliferation, contribute to the effects of DBcAMP on the healing of skin ulcers.

• Authors: L J Zhou, I Ono
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 143 Issue 3 Pg. 506-12 (Sep 2000) ISSN: 0007-0963 [Print] England
• PMID: 10971321 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Interleukin-6
• Transforming Growth Factor beta
• Bucladesine

Topics

• Adolescent
• Bucladesine (pharmacology)
• Cell Division (drug effects)
• Cells, Cultured (drug effects)
• Cytokines (biosynthesis)
• Fibroblasts (drug effects, metabolism)
• Humans
• Interleukin-6 (metabolism)
• Keratinocytes (cytology, drug effects, metabolism)
• Transforming Growth Factor beta (metabolism)
• Wound Healing (drug effects)