Abstract |
Coke oven workers are often heavily exposed to polynuclear aromatic hydrocarbons (PAHs) and particularly to benzo[a]pyrene (B[a]P), which has been associated with a high incidence of cancer. B[a]P is metabolically activated to its diol- epoxide derivative, benzo[a]pyrene-trans-7,8- dihydrodiol-9,10- epoxide ( BPDE-I), a potent carcinogen which binds covalently to DNA, thereby producing BPDE-I- DNA adducts. In this study, an investigation was made of the exposure of coke oven workers to PAH via the measurement of urinary 1-hydroxypyrene (1-OHP) levels, and of exposure to B[a]P by the analysis of BPDE-I- DNA adducts in leukocytes using an ELISA competitive immunoassay. 1-OHP levels measured in post-shift samples correlated with those of DNA adducts detected in leukocytes, with a mean value (140.11 fM/50 micrograms of DNA) which differed significantly from the control value (P < 0.001). It is concluded that measurement of BPDE-I- DNA adduct levels in coke plant workers is essential in determining cancer risk due to high exposure to PAHs, and in particular of B[a]P.
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Authors | J P Arnould, R Kubiak, J Belowski, J Belegaud, J Szczeklik |
Journal | Pathologie-biologie
(Pathol Biol (Paris))
Vol. 48
Issue 6
Pg. 548-53
(Jul 2000)
ISSN: 0369-8114 [Print] France |
PMID | 10965532
(Publication Type: Journal Article)
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Chemical References |
- Air Pollutants, Occupational
- Benzopyrenes
- Biomarkers
- Coke
- Polycyclic Compounds
- Pyrenes
- benzo(a)pyrene diolepoxide I
- 1-hydroxypyrene
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Topics |
- Air Pollutants, Occupational
(adverse effects)
- Benzopyrenes
(analysis)
- Biomarkers
- Coke
- DNA Damage
- Humans
- Leukocytes
(chemistry)
- Male
- Occupational Exposure
- Poland
- Polycyclic Compounds
(adverse effects)
- Pyrenes
(analysis)
- Smoking
(epidemiology)
- Urine
(chemistry)
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