Abstract |
The extracellular homeostasis of glutamate in the brain is maintained by the efficient uptake into astroglial cells. The high extracellular glutamate levels seen during seizures are therefore probably a result of both an increased synaptic release and a deranged glutamate uptake. In this study we used immuno-blotting technique to measure the cortical levels of the astrocytic glutamate transport protein (GLT-1) and of the glutamate and aspartate transporting protein (GLAST) in an epilepsy model induced by ferrous chloride injection in the cortex of rats. The levels of GLT-1 were lower in epileptic rats than in controls, day 1 and 5 after induction, but not at 3 months. Glial fibrillary protein (GFAP) levels increased with time in the epileptic model, whereas GLAST and beta-tubulin III remained unchanged compared to controls. The results suggest that the transient decrease of GLT-1 could play a role in epileptogenesis, while recurrent seizure activity may be maintained by other mechanisms.
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Authors | C Samuelsson, E Kumlien, R Flink, D Lindholm, E Ronne-Engström |
Journal | Neuroscience letters
(Neurosci Lett)
Vol. 289
Issue 3
Pg. 185-8
(Aug 11 2000)
ISSN: 0304-3940 [Print] Ireland |
PMID | 10961660
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP-Binding Cassette Transporters
- Amino Acid Transport System X-AG
- Ferrous Compounds
- Glial Fibrillary Acidic Protein
- Tubulin
- Glutamic Acid
- ferrous chloride
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Topics |
- ATP-Binding Cassette Transporters
(metabolism)
- Amino Acid Transport System X-AG
- Animals
- Astrocytes
(drug effects, metabolism, pathology)
- Cerebral Cortex
(metabolism, pathology, physiopathology)
- Disease Models, Animal
- Electroencephalography
(drug effects)
- Epilepsy, Post-Traumatic
(chemically induced, metabolism, physiopathology)
- Ferrous Compounds
(adverse effects)
- Glial Fibrillary Acidic Protein
(metabolism)
- Glutamic Acid
(metabolism)
- Male
- Neurons
(drug effects, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
- Tubulin
(metabolism)
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