Immune system activation by CDDP and "poly-plat".

Abstract

Murine peritoneal macrophages, human fibroblasts and human ovarian teratocarcinoma cells were treated with CDDP (10 micrograms/ml) and "poly-plat" (10 micrograms/ml) for 2 h and cultured for 2-96 h. After "poly-plat" treatment macrophages developed cytoplasmic extensions much faster and secreted higher levels of interleukin-2 (IL-2), compared to CDDP. "Poly-plat" treated human fibroblasts demonstrated a significant release of IL-2 (138 pg/ml), compared to CDDP (64 pg/ml) or the untreated normal cells (31 pg/ml) after 8 h. Correspondingly there was a 53% increase in cells after "poly-plat" treatment compared to only 37% after CDDP. However, human ovarian teratocarcinoma cells didn't show any significant increase in IL-2 levels after "poly-plat" treatment and there was instead a decrease of 15% in cell numbers at 8 h. Based on our studies, we propose that "poly-plat" is more effective in activation of the macrophages and human fibroblasts in terms of IL-2, but not so with human ovarian teratocarcinoma cells.

• Authors: K Burkitt, S K Aggarwal
• Journal: Anticancer research (Anticancer Res) 2000 Jul-Aug Vol. 20 Issue 4 Pg. 2729-37 ISSN: 0250-7005 [Print] Greece
• PMID: 10953351 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Antineoplastic Agents
• Interleukin-2
• Organoplatinum Compounds
• poly(trans-1,2-diaminocyclohexane)platinum-carboxyamylose
• Cisplatin

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Antineoplastic Agents (pharmacology)
• Cisplatin (pharmacology)
• Female
• Fibroblasts (drug effects)
• Humans
• Interleukin-2 (metabolism)
• Macrophage Activation (drug effects)
• Macrophages, Peritoneal (drug effects, pathology)
• Mice
• Organoplatinum Compounds (pharmacology)
• Ovarian Neoplasms (pathology)
• Teratocarcinoma (pathology)