HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Fanconi anemia complementation group C is required for proliferation of murine primordial germ cells.

Abstract
Fanconi anemia is a polygenic trait hypothesized to be a DNA damage repair disease. We show that all three Fanconi anemia loci that have been cloned are expressed in the embryonic gonad during the period of primordial germ cell proliferation. Mice mutant for the Fanconi anemia complementation group C locus (Fancc) have reduced germ cell numbers as early as embryonic day E12.5, suggesting the Fancc protein functions prior to meiosis in both sexes. Depletion in the mutant occurs at a time when all three loci would be expressed in a wild-type gonad, implying a function in the early germline. Determination of the mitotic index of primordial germ cells by BrdU incorporation shows that germ cells in Fancc(-/-) mice proliferate significantly more slowly than littermate controls. This study demonstrates Fancc is required for mitotic proliferation of primordial germ cells.
AuthorsJ J Nadler, R E Braun
JournalGenesis (New York, N.Y. : 2000) (Genesis) Vol. 27 Issue 3 Pg. 117-23 (Jul 2000) ISSN: 1526-954X [Print] United States
PMID10951504 (Publication Type: Letter, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fancc protein, mouse
  • Fanconi Anemia Complementation Group C Protein
  • Fanconi Anemia Complementation Group Proteins
  • Nuclear Proteins
  • Proteins
Topics
  • Animals
  • Cell Cycle Proteins
  • Cell Division
  • DNA-Binding Proteins
  • Embryonic and Fetal Development (genetics)
  • Fanconi Anemia (genetics)
  • Fanconi Anemia Complementation Group C Protein
  • Fanconi Anemia Complementation Group Proteins
  • Female
  • Gene Deletion
  • Male
  • Meiosis
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mitotic Index
  • Nuclear Proteins
  • Ovary (embryology)
  • Ovum (cytology, physiology)
  • Pregnancy
  • Proteins (genetics, metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spermatozoa (cytology, physiology)
  • Testis (embryology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: