Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.

Abstract

Missile therapy, which destroys cancer cells specifically, has been regarded as an effective treatment modality for carcinoma. The monoclonal antibody MSN-1 (IgM), which reacts strongly with endometrial adenocarcinomas, was combined with adriamycin (ADM) by a disulfide bond using N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP) and 2-iminothiolane. Its selective cytotoxicity against SNG-II was examined in a colony formation in vitro, and on athymic mice in vivo. The results of our study suggest that the <inhibitory concentration> or IC50, of the MSN-1-ADM immunoconjugate against SNG-II to be 57 times that of ADM alone in vitro. The reductions in resected weights of target tumor cells, at the local site of the MSN-1-ADM immunoconjugate treatment, were 25% with caudal vein administration, and 38% with local administration, as compared with the untreated group, in vivo. There was no weight loss in treated mice. Our results suggest that this MSN-1-ADM immunoconjugate has potential clinical application in the treatment of endometrial adenocarcinomas.

• Authors: Y Kaneta, K Tsukazaki, K Kubushiro, M Sakayori, M Ueda, S Nozawa
• Journal: Oncology reports (Oncol Rep) 2000 Sep-Oct Vol. 7 Issue 5 Pg. 1099-106 ISSN: 1021-335X [Print] Greece
• PMID: 10948346 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antineoplastic Agents
• Immunoglobulin M
• Immunotoxins
• Doxorubicin

Topics

• Adenocarcinoma (drug therapy, immunology)
• Animals
• Antibodies, Monoclonal (immunology, metabolism)
• Antineoplastic Agents (antagonists & inhibitors, toxicity)
• Binding, Competitive
• Dose-Response Relationship, Drug
• Doxorubicin (antagonists & inhibitors, toxicity)
• Endometrial Neoplasms (drug therapy, immunology)
• Female
• Flow Cytometry
• Humans
• Immunoglobulin M (immunology, metabolism)
• Immunotoxins (chemistry, immunology, toxicity)
• Inhibitory Concentration 50
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Spectrometry, Fluorescence
• Tumor Cells, Cultured