Abstract |
A quick and reproducible method for radiolabeling of Photosan-3(R), a photosensitizer used worldwide for photodynamic therapy ( PDT) of cancer, with radioisotope of technetium ((99m)Tc) was developed. The radiotracer was evaluated for radiochemical purity, stability, and tissue distribution in a murine tumor model. The (99m)Tc-Photosan-3, which was prepared by using (99m)Tc-pertechnetate in place of reduced (99m)Tc, demonstrated better labeling efficiency (>90%) and reproducibility. The procedure also minimized radiation exposure to the radiochemist because handling time was considerably reduced. Due to the commercial availability of Photosan-3, the risk of batch-to-batch variation in the in situ synthesis of hematoporphyrin derivative, which is a complex mixture of at least five compounds, was also significantly reduced. The biodistribution studies and tumor scintigraphy confirmed that (99m)Tc-labeled Photosan-3 was preferentially taken up by the neoplastic tissue similar to the parent compound. In addition to its applications in tumor imaging, (99m)Tc-Photosan-3 could also be used for estimating tumor uptake of Photosan-3 as may be required for individualization of clinical protocols of PDT.
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Authors | A K Babbar, A K Singh, H C Goel, U P Chauhan, R K Sharma |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 27
Issue 4
Pg. 419-26
(May 2000)
ISSN: 0969-8051 [Print] United States |
PMID | 10938479
(Publication Type: Journal Article)
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Chemical References |
- Hematoporphyrins
- Photosensitizing Agents
- Radiopharmaceuticals
- photosan III
- Technetium
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Topics |
- Animals
- Arboviruses
- Drug Stability
- Hematoporphyrins
- Isotope Labeling
- Neoplasms, Experimental
(diagnostic imaging)
- Photosensitizing Agents
(chemistry, pharmacokinetics)
- Protein Binding
- Rabbits
- Radionuclide Imaging
- Radiopharmaceuticals
- Technetium
- Tissue Distribution
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