Abstract | PURPOSE: To determine the in vitro drug susceptibility of Australian corneal isolates of Acanthamoeba and to correlate the results with patient treatment and visual outcome. METHODS: Acanthomoeba isolates were obtained from a reference laboratory Cyst suspensions were prepared from 19 strains and exposed to 10 antimicrobial agents for 7 days. The minimum drug concentrations required to inhibit excystation were determined. Inhibited cells were then plated out to determine minimum cysticidal concentrations. RESULTS: CONCLUSIONS:
Propamidine and polyhexamethylene biguanide drops are recommended as initial choices for the treatment of Acanthamoeba keratitis. The variability in the susceptibility to any one agent suggests that individual testing of isolates is necessary to identify the most appropriate treatment. A number of factors influence visual outcome in these cases; further studies are required to resolve the importance or otherwise of in vitro susceptibility.
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Authors | L Lim, D J Coster, P R Badenoch |
Journal | Clinical & experimental ophthalmology
(Clin Exp Ophthalmol)
Vol. 28
Issue 2
Pg. 119-24
(Apr 2000)
ISSN: 1442-6404 [Print] Australia |
PMID | 10933775
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Anti-Infective Agents, Local
- Antiprotozoal Agents
- Disinfectants
- Ophthalmic Solutions
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Topics |
- Acanthamoeba
(drug effects, isolation & purification)
- Acanthamoeba Keratitis
(drug therapy, parasitology)
- Adult
- Animals
- Anti-Infective Agents, Local
(pharmacology)
- Antiprotozoal Agents
(pharmacology)
- Disinfectants
(pharmacology)
- Female
- Humans
- Incidence
- Male
- Middle Aged
- Ophthalmic Solutions
- Parasitic Sensitivity Tests
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