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Arylamine N-acetyltransferase activity and 2 aminofluorene-DNA adducts formation in rat glial tumor cells.

Abstract
Arylamine N-acetylation capacity by the N-acetyltransferase (NAT) may be an important causative factor in the initiation of cancer. Arylamine-DNA adducts formation have been correlated with the carcinogenic effect of heterocyclic aromatic amines. NAT activity in rat glial tumor cells was measured by high performance liquid chromatography (HPLC) using 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA) as substrares. 2-AF-DNA adducts formation in rat glial tumor cells was investigated by gamma-[32p]-dATP and HPLC using 2-aminofluorene as substrates. The activities (Mean +/- SD) of NAT in rat glial cells was 1.08 +/- 0.18 nmol/min/mg protein for the acetylation of 2-aminofluorene (n = 12), and 0.96 +/- 0.16 nmol/min/mg protein for the acetylation of p-aminobenzoic acid (n = 12). 2-AF-DNA adducts formation in rat glial tumor cells with 30 microM and 60 microM AF were 0.48 +/- 0.16 and 0.70 +/- 0.12 pmol/mg DNA, respectively. The results indicate that NAT was present in rat glial tumor cells, activating AF to become a metabolite able to bind covalently with DNA to form 2-AF-DNA.
AuthorsC F Hung, K T Wong, C I Tsai, C S Lin, J G Chung
JournalActa paediatrica Taiwanica = Taiwan er ke yi xue hui za zhi (Acta Paediatr Taiwan) 2000 May-Jun Vol. 41 Issue 3 Pg. 133-5 ISSN: 1608-8115 [Print] China (Republic : 1949- )
PMID10920545 (Publication Type: Journal Article)
Chemical References
  • DNA Adducts
  • Fluorenes
  • Mutagens
  • 2-aminofluorene
  • Arylamine N-Acetyltransferase
Topics
  • Animals
  • Arylamine N-Acetyltransferase (analysis)
  • Brain Neoplasms (chemistry)
  • Cell Transformation, Neoplastic (chemistry)
  • Chromatography, High Pressure Liquid
  • DNA Adducts (analysis)
  • Fluorenes (analysis)
  • Glioma (chemistry)
  • Mutagens (analysis)
  • Rats
  • Tumor Cells, Cultured (chemistry)

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