Abstract |
Arylamine N-acetylation capacity by the N- acetyltransferase ( NAT) may be an important causative factor in the initiation of cancer. Arylamine- DNA adducts formation have been correlated with the carcinogenic effect of heterocyclic aromatic amines. NAT activity in rat glial tumor cells was measured by high performance liquid chromatography (HPLC) using 2-aminofluorene (2-AF) and p-aminobenzoic acid ( PABA) as substrares. 2-AF-DNA adducts formation in rat glial tumor cells was investigated by gamma-[32p]-dATP and HPLC using 2-aminofluorene as substrates. The activities (Mean +/- SD) of NAT in rat glial cells was 1.08 +/- 0.18 nmol/min/mg protein for the acetylation of 2-aminofluorene (n = 12), and 0.96 +/- 0.16 nmol/min/mg protein for the acetylation of p-aminobenzoic acid (n = 12). 2-AF-DNA adducts formation in rat glial tumor cells with 30 microM and 60 microM AF were 0.48 +/- 0.16 and 0.70 +/- 0.12 pmol/mg DNA, respectively. The results indicate that NAT was present in rat glial tumor cells, activating AF to become a metabolite able to bind covalently with DNA to form 2-AF-DNA.
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Authors | C F Hung, K T Wong, C I Tsai, C S Lin, J G Chung |
Journal | Acta paediatrica Taiwanica = Taiwan er ke yi xue hui za zhi
(Acta Paediatr Taiwan)
2000 May-Jun
Vol. 41
Issue 3
Pg. 133-5
ISSN: 1608-8115 [Print] China (Republic : 1949- ) |
PMID | 10920545
(Publication Type: Journal Article)
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Chemical References |
- DNA Adducts
- Fluorenes
- Mutagens
- 2-aminofluorene
- Arylamine N-Acetyltransferase
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Topics |
- Animals
- Arylamine N-Acetyltransferase
(analysis)
- Brain Neoplasms
(chemistry)
- Cell Transformation, Neoplastic
(chemistry)
- Chromatography, High Pressure Liquid
- DNA Adducts
(analysis)
- Fluorenes
(analysis)
- Glioma
(chemistry)
- Mutagens
(analysis)
- Rats
- Tumor Cells, Cultured
(chemistry)
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