The human cytomegalovirus 86-kilodalton major immediate-early protein interacts physically and functionally with histone acetyltransferase P/CAF.

Abstract	The major immediate-early proteins of human cytomegalovirus (HCMV) play a pivotal role in controlling viral and cellular gene expression during productive infection. As well as negatively autoregulating its own promoter, the HCMV 86-kDa major immediate early protein (IE86) activates viral early gene expression and is known to be a promiscuous transcriptional regulator of cellular genes. IE86 appears to act as a multimodal transcription factor. It is able to bind directly to target promoters to activate transcription but is also able to bridge between upstream binding factors such as CREB/ATF and the basal transcription complex as well as interacting directly with general transcription factors such as TATA-binding protein and TFIIB. We now show that IE86 is also able to interact directly with histone acetyltransferases during infection. At least one of these factors is the histone acetyltransferase CBP-associated factor (P/CAF). Furthermore, we show that this interaction results in synergistic transactivation by IE86 of IE86-responsive promoters. Recruitment of such chromatin-remodeling factors to target promoters by IE86 may help explain the ability of this viral protein to act as a promiscuous transactivator of cellular genes.
Authors	L A Bryant, P Mixon, M Davidson, A J Bannister, T Kouzarides, J H Sinclair
Journal	Journal of virology (J Virol) Vol. 74 Issue 16 Pg. 7230-7 (Aug 2000) ISSN: 0022-538X [Print] United States
PMID	10906177 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Cell Cycle Proteins IE2 protein, Cytomegalovirus Immediate-Early Proteins Membrane Glycoproteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors UL115 protein, Human herpesvirus 5 Viral Envelope Proteins Viral Proteins glycoprotein H, Cytomegalovirus glycoprotein H, Human cytomegalovirus glycoprotein O, cytomegalovirus Acetyltransferases Histone Acetyltransferases p300-CBP Transcription Factors p300-CBP-associated factor Glutathione Transferase
Topics	Acetyltransferases (genetics, metabolism) Cell Cycle Proteins (genetics, metabolism) Cytomegalovirus (genetics, physiology) Cytomegalovirus Infections (virology) Gene Expression Regulation Glutathione Transferase (genetics, metabolism) Histone Acetyltransferases Humans Immediate-Early Proteins (genetics, metabolism) Membrane Glycoproteins Precipitin Tests Recombinant Fusion Proteins (genetics, metabolism) Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Transcriptional Activation Transfection Tumor Cells, Cultured Two-Hybrid System Techniques Viral Envelope Proteins Viral Proteins p300-CBP Transcription Factors

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