HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Adenovirus-mediated gene transfer into an experimental pancreatic tumour.

AbstractBACKGROUND AND AIMS:
Gene therapy has been suggested as a novel approach against pancreatic cancer, a disease with a grim prognosis with current modes of therapy. Despite recent advances in in vitro and experimental in vivo studies, there is no data available concerning gene transfer efficiency in intrapancreatic tumours in immunocompetent animal models.
MATERIAL AND METHODS:
In in vitro studies rat pancreatic carcinoma cells (DSL-6A/C1) were transduced with replication-deficient adenovirus carrying Escherichia Coli beta-galactosidase (lacZ) gene. Gene transfer efficacy was assessed at different multiplicities of infection (MOIs). Pancreatic tumours were induced by inoculating cultured DSL-6A/C1 cancer cells into Lewis rat pancreases. Established tumours were transduced and three days post-transduction, pancreatic tumours as well as other intra-abdominal organs were harvested and processed for histological analyses, including staining for marker gene expression.
RESULTS AND CONCLUSIONS:
In vitro assays showed that DSL-6A/C1 cells were transduced efficiently, even at low MOIs. In vivo gene transfer was successful in all animals, and all pancreatic samples showed reporter gene expression. Positive cells were detected in the peritumoural areas as well as to a lesser extent within the tumours. The transgene activity was not evenly distributed and the gene transfer efficiency varied from a few detectable blue cells to 11% per field. Our studies demonstrated safe in vivo gene transfer into intrapancreatic tumours, suggesting that pancreatic tumours are potential targets for in vivo delivery of therapeutic genes.
AuthorsK Mäkinen, S Loimas, V M Kosma, J Wahlfors, S Ylä-Herttuala, E Alhava, J Jänne
JournalAnnales chirurgiae et gynaecologiae (Ann Chir Gynaecol) Vol. 89 Issue 2 Pg. 99-103 ( 2000) ISSN: 0355-9521 [Print] FINLAND
PMID10905674 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • beta-Galactosidase
Topics
  • Adenoviruses, Human
  • Animals
  • Carcinoma, Pancreatic Ductal (therapy)
  • Disease Models, Animal
  • Gene Expression Regulation, Neoplastic
  • Genetic Therapy
  • Genetic Vectors
  • Immunocompetence
  • Male
  • Pancreatic Neoplasms (therapy)
  • Rats
  • Rats, Inbred Lew
  • Tumor Cells, Cultured
  • beta-Galactosidase (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: