Abstract |
To evaluate a long peptide-based allergy vaccine in a murine model, CBA/J mice were sensitized with low dose alum-adsorbed phospholipase A2 (PLA2), a major bee venom allergen. Presensitized mice were treated by daily i.p. injections of a mixture of three long overlapping peptides (44- to 60-mer) spanning the entire PLA2 molecule (100 microg/ peptide) for 6 consecutive days. This therapeutic approach induced a sharp drop in PLA2-specific IgE, an increase in specific IgG2a, and a marked T cell hyporesponsiveness. T cell cytokine secretion was characterized by a shift from a Th2 to a Th1 profile. Prophylactic treatment of naive mice with long peptides prior to sensitization with PLA2 induced a comparable modulation of B and T cell responses. Upon i.p. challenge with native PLA2, presensitized mice treated with the long peptide mixture were fully protected from anaphylaxis. This indicated that allergen-derived long overlapping peptides were safe and able to modulate an established Th2 response or to prevent its development. Furthermore, long peptide-based immunotherapy provided clinical protection against anaphylaxis, thus appearing as a promising approach of the therapy of allergic diseases.
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Authors | C von Garnier, M Astori, A Kettner, N Dufour, C Heusser, G Corradin, F Spertini |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 30
Issue 6
Pg. 1638-45
(Jun 2000)
ISSN: 0014-2980 [Print] Germany |
PMID | 10898500
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allergens
- Bee Venoms
- Immunoglobulin G
- Peptides
- Immunoglobulin E
- Phospholipases A
- Phospholipases A2
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Topics |
- Allergens
(administration & dosage, immunology)
- Anaphylaxis
(prevention & control)
- Animals
- Bee Venoms
(administration & dosage, chemical synthesis, immunology)
- Cell Division
- Down-Regulation
(immunology)
- Female
- Immunoglobulin E
(blood)
- Immunoglobulin G
(blood)
- Immunotherapy
- Injections, Intraperitoneal
- Mice
- Mice, Inbred CBA
- Peptides
(administration & dosage, chemical synthesis, immunology)
- Phospholipases A
(administration & dosage, chemical synthesis, immunology)
- Phospholipases A2
- T-Lymphocytes
(immunology)
- Th1 Cells
(immunology)
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