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Allergen-derived long peptide immunotherapy down-regulates specific IgE response and protects from anaphylaxis.

Abstract
To evaluate a long peptide-based allergy vaccine in a murine model, CBA/J mice were sensitized with low dose alum-adsorbed phospholipase A2 (PLA2), a major bee venom allergen. Presensitized mice were treated by daily i.p. injections of a mixture of three long overlapping peptides (44- to 60-mer) spanning the entire PLA2 molecule (100 microg/peptide) for 6 consecutive days. This therapeutic approach induced a sharp drop in PLA2-specific IgE, an increase in specific IgG2a, and a marked T cell hyporesponsiveness. T cell cytokine secretion was characterized by a shift from a Th2 to a Th1 profile. Prophylactic treatment of naive mice with long peptides prior to sensitization with PLA2 induced a comparable modulation of B and T cell responses. Upon i.p. challenge with native PLA2, presensitized mice treated with the long peptide mixture were fully protected from anaphylaxis. This indicated that allergen-derived long overlapping peptides were safe and able to modulate an established Th2 response or to prevent its development. Furthermore, long peptide-based immunotherapy provided clinical protection against anaphylaxis, thus appearing as a promising approach of the therapy of allergic diseases.
AuthorsC von Garnier, M Astori, A Kettner, N Dufour, C Heusser, G Corradin, F Spertini
JournalEuropean journal of immunology (Eur J Immunol) Vol. 30 Issue 6 Pg. 1638-45 (Jun 2000) ISSN: 0014-2980 [Print] Germany
PMID10898500 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Allergens
  • Bee Venoms
  • Immunoglobulin G
  • Peptides
  • Immunoglobulin E
  • Phospholipases A
  • Phospholipases A2
Topics
  • Allergens (administration & dosage, immunology)
  • Anaphylaxis (prevention & control)
  • Animals
  • Bee Venoms (administration & dosage, chemical synthesis, immunology)
  • Cell Division
  • Down-Regulation (immunology)
  • Female
  • Immunoglobulin E (blood)
  • Immunoglobulin G (blood)
  • Immunotherapy
  • Injections, Intraperitoneal
  • Mice
  • Mice, Inbred CBA
  • Peptides (administration & dosage, chemical synthesis, immunology)
  • Phospholipases A (administration & dosage, chemical synthesis, immunology)
  • Phospholipases A2
  • T-Lymphocytes (immunology)
  • Th1 Cells (immunology)

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