Prevalence of beta-lactamase-producing strains among 149 anaerobic gram-negative rods isolated from periodontal pockets.

Abstract

In a prospective study, 47 adults presenting a rapidly progressive periodontitis were selected in order to evaluate the prevalence of beta-lactamase-producing strains among oral anaerobic gram-negative rods. Predominant anaerobes were identified from two of the deepest periodontal pockets. beta-Lactamase-positive strains fulfilled to at least two of three criteria: positive nitrocefin test, penicillin Etest minimal inhibitory concentration > 1 microgram/ml, and disk diffusion synergy between amoxycillin and clavulanic acid > 10 mm. At least one beta-lactamase-producing strain was found in 53.2% of patients and 39.4% of the periodontal pockets investigated. Prominent beta-lactamase-positive species were Prevotella buccae and Prevotella intermedia (respectively 16 of 38: 42% and 18 of 52: 35% positive strains), followed by Prevotella bivia, Prevotella disiens, Prevotella denticola and Fusobacterium nucleatum (respectively 1 of 6: 17%, 1 of 10: 10%, 1 of 10: 10%, and 1 of 13: 8% positive strains). No beta-lactamase producer could be evidenced in Porphyromonas gingivalis (10 strains tested). All the beta-lactamase-positive strains with the nitrocefin test had penicillin minimal inhibitory concentrations > 1 microgram/ml with the Etest, and a strong synergy between amoxicillin and clavulanic acid was always observed.

• Authors: T Fosse, I Madinier, C Hitzig, Y Charbit
• Journal: Oral microbiology and immunology (Oral Microbiol Immunol) Vol. 14 Issue 6 Pg. 352-7 (Dec 1999) ISSN: 0902-0055 [Print] Denmark
• PMID: 10895690 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• beta-Lactamases

Topics

• Adult
• Bacterial Proteins (biosynthesis)
• Fusobacterium nucleatum (enzymology)
• Gram-Negative Anaerobic Straight, Curved, and Helical Rods (enzymology)
• Humans
• Microbial Sensitivity Tests
• Penicillin Resistance
• Periodontal Pocket (microbiology)
• Prevotella (enzymology)
• Prospective Studies
• beta-Lactamases (biosynthesis)