Expression of N-myristoyltransferase inhibitor protein and its relationship to c-Src levels in human colon cancer cell lines.

Abstract

Earlier, we have reported that N-myristoyltransferase (NMT) activity is higher in colonic epithelial neoplasms than in normal appearing colonic tissue and that increase in NMT activity appears at an early stage in colonic carcinogenesis [Magnuson, B., Raju, R. V. S., Moyana, T. N., and Sharma, R. K. (1995) J. Natl. Cancer Inst. 87, 1630-1635]. In this study, we demonstrate increased NMT mRNA in well-differentiated adenocarcinomas. NMT and c-Src mRNA levels were generally elevated in a subset of human colon cancer cell lines. Western blotting analysis employing N-myristoyltransferase inhibitory protein (NIP(71)) antibody demonstrated low levels of NIP(71) in high-expressing c-Src cell lines and high levels of NIP(71) in low-expressing c-Src cell lines. Interestingly, down regulation of c-Src by antisense expression in the HT-29 cell line resulted in increased expression of NIP(71), suggesting c-Src may negatively regulate NIP(71) expression. Furthermore, this is the first study demonstrating the expression of NIP(71) in human colon cancer cell lines and a possible relationship to colon carcinogenesis.

• Authors: R V Rajala, S Dehm, X Bi, K Bonham, R K Sharma
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 273 Issue 3 Pg. 1116-20 (Jul 14 2000) ISSN: 0006-291X [Print] United States
• PMID: 10891381 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Enzyme Inhibitors
• RNA, Messenger
• Acyltransferases
• glycylpeptide N-tetradecanoyltransferase
• Proto-Oncogene Proteins pp60(c-src)

Topics

• Acyltransferases (antagonists & inhibitors, genetics, metabolism)
• Animals
• Blotting, Northern
• Colonic Neoplasms (metabolism)
• Enzyme Inhibitors (metabolism)
• Humans
• Proto-Oncogene Proteins pp60(c-src) (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rabbits
• Tumor Cells, Cultured