HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

In situ photoaffinity labeling of proteasome with photoactive adriamycin analogue.

Abstract
An intracellular adriamycin (ADM)-binding protein purified from the cytosol of L1210 mouse lymphocytic leukemia cells had a molecular weight of 700-1500 kDa and hydrolyzed Suc-LLVY-MCA. When L1210 cells were incubated with a photoactive ADM analogue, N-(p-azidobenzoyl)-adriamycin (NAB-ADM), most of the NAB-ADM was found to localize in the nuclei. In situ photoaffinity labeling of L1210 cells with NAB-ADM resulted in low protease activity in the cytosol and nuclear extracts and the cells showed selective photoincorporation of NAB-ADM into the proteasome. These results suggest that the proteasome is a translocator of ADM from the cytoplasm to the nucleus and might therefore become a new candidate for cancer chemotherapy.
AuthorsK I Kiyomiya, S Matsuo, M Kurebe
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 273 Issue 3 Pg. 928-32 (Jul 14 2000) ISSN: 0006-291X [Print] United States
PMID10891349 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2000 Academic Press.
Chemical References
  • Multienzyme Complexes
  • Photoaffinity Labels
  • Doxorubicin
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
Topics
  • Animals
  • Cysteine Endopeptidases (chemistry, isolation & purification)
  • Doxorubicin (analogs & derivatives, chemistry)
  • Electrophoresis, Polyacrylamide Gel
  • Leukemia L1210 (pathology)
  • Mice
  • Multienzyme Complexes (chemistry, isolation & purification)
  • Photoaffinity Labels
  • Proteasome Endopeptidase Complex
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: