Effect of the sialyl Lewis X (SLe(x)) moiety on splenic accumulation of SLe(x)-carboxymethylpullulan conjugate.

Abstract

Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CMPul was modified with a saccharide unable to bind to E-selectin, this splenic accumulation was not observed. The uptake of radiolabeled SLe(x)-CMPul by the spleen was completely inhibited by a 100-fold molar of cold SLe(x)-CMPul but not by a sialyl N-acetyllactosamine-CMPul conjugate (SLN-CMPul). Microautoradiography analyses revealed that SLe(x)-CMPul accumulated in the marginal zone of the spleen.

• Authors: K Horie, M Sakagami, K Kuramochi, T Ito, H Hamana
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 44 Issue 5 Pg. 401-4 ( 2000) ISSN: 0385-5600 [Print] Australia
• PMID: 10888360 (Publication Type: Journal Article)

Chemical References

• E-Selectin
• Glucans
• Oligosaccharides
• Sialyl Lewis X Antigen
• carboxymethylpullulan
• Arachidonic Acid

Topics

• Animals
• Arachidonic Acid
• E-Selectin (metabolism)
• Edema (chemically induced, metabolism)
• Glucans (chemistry, metabolism, pharmacokinetics)
• Injections, Intravenous
• Lung (metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Oligosaccharides (chemistry, metabolism, pharmacokinetics)
• Sialyl Lewis X Antigen
• Spleen (metabolism)
• Tissue Distribution